NDLI: Proteomics-Identified Bvg-Activated Autotransporters Protect against Bordetella pertussis in a Mouse Model

Content Provider	PubMed Central
Author	Gouw, Daan De Jonge, Marien I. De Hermans, Peter W. M. Wessels, Hans J. C. T. Zomer, Aldert Berends, Alinda Pratt, Catherine Berbers, Guy A. Mooi, Frits R. Diavatopoulos, Dimitri A.
Editor	Miyaji, Eliane Namie
Copyright Year	2014
Abstract	Pertussis is a highly infectious respiratory disease of humans caused by the bacterium Bordetella pertussis. Despite high vaccination coverage, pertussis has re-emerged globally. Causes for the re-emergence of pertussis include limited duration of protection conferred by acellular pertussis vaccines (aP) and pathogen adaptation. Pathogen adaptations involve antigenic divergence with vaccine strains, the emergence of strains which show enhanced in vitro expression of a number of virulence-associated genes and of strains that do not express pertactin, an important aP component. Clearly, the identification of more effective B. pertussis vaccine antigens is of utmost importance. To identify novel antigens, we used proteomics to identify B. pertussis proteins regulated by the master virulence regulatory system BvgAS in vitro. Five candidates proteins were selected and it was confirmed that they were also expressed in the lungs of naïve mice seven days after infection. The five proteins were expressed in recombinant form, adjuvanted with alum and used to immunize mice as stand-alone antigens. Subsequent respiratory challenge showed that immunization with the autotransporters Vag8 and SphB1 significantly reduced bacterial load in the lungs. Whilst these antigens induced strong opsonizing antibody responses, we found that none of the tested alum-adjuvanted vaccines - including a three-component aP - reduced bacterial load in the nasopharynx, suggesting that alternative immunological responses may be required for efficient bacterial clearance from the nasopharynx.
Related Links	http://dx.doi.org/10.1371/journal.pone.0105011
Starting Page	105011
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	8
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-08-18
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Correction: Proteomics-Identified Bvg-Activated Autotransporters Protect against Bordetella pertussis in a Mouse Model

Overexpression of the RNA Polymerase Alpha Subunit Reduces Transcription of Bvg-Activated Virulence Genes in Bordetella pertussis

Positive transcriptional feedback at the bvg locus controls expression of virulence factors in Bordetella pertussis.

Positive transcriptional feedback at the bvg locus controls expression of virulence factors in Bordetella pertussis

Vag8, a Bordetella pertussis bvg-Regulated Protein

The Role of Virulence Proteins in Protection Conferred by Bordetella pertussis Outer Membrane Vesicle Vaccines

Differential Regulation of Bvg-Activated Virulence Factors Plays a Role in Bordetella pertussis Pathogenicity

Bordetella pertussis TonB, a Bvg-Independent Virulence Determinant

Identification of a Bordetella pertussis bvg-regulated porin-like protein.

Proteomics-Identified Bvg-Activated Autotransporters Protect against Bordetella pertussis in a Mouse Model

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Correction: Proteomics-Identified Bvg-Activated Autotransporters Protect against Bordetella pertussis in a Mouse Model

Overexpression of the RNA Polymerase Alpha Subunit Reduces Transcription of Bvg-Activated Virulence Genes in Bordetella pertussis

Positive transcriptional feedback at the bvg locus controls expression of virulence factors in Bordetella pertussis.

Positive transcriptional feedback at the bvg locus controls expression of virulence factors in Bordetella pertussis

Vag8, a Bordetella pertussis bvg-Regulated Protein

The Role of Virulence Proteins in Protection Conferred by Bordetella pertussis Outer Membrane Vesicle Vaccines

Differential Regulation of Bvg-Activated Virulence Factors Plays a Role in Bordetella pertussis Pathogenicity

Bordetella pertussis TonB, a Bvg-Independent Virulence Determinant

Identification of a Bordetella pertussis bvg-regulated porin-like protein.

Proteomics-Identified Bvg-Activated Autotransporters Protect against Bordetella pertussis in a Mouse Model