NDLI: Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro

Content Provider	PubMed Central
Author	Sadegh, Mohajer Taha, Rosna Mat Mohajer, Minoo Esmaeili, Arash Khorasani
Copyright Year	2014
Abstract	To explore the potential of in vitro rapid regeneration, three varieties (Golpaygan-181, Orumieh-1763, and Gorgan-1601) of sainfoin (Onobrychis viciifolia Scop. syn. Onobrychis sativa L.) were evaluated. For the first time, an encapsulation protocol was established from somatic embryogenic callus in torpedo and cotyledonary stages to create artificial seeds. Callus derived from different concentrations of Kinetin (0–2.0 mg L−1) and Indole-3-acetic acid (0–2.0 mg L−1) was coated with sodium alginate and subsequently cultured either in Murashige and Skoog (MS) medium or in soil substrate. Adventitious shoots from synthetic beads developed into rooting in full and half strength MS medium supplemented with various concentrations of auxin and cytokinin. Prolonged water conservation of black and red soils (1 : 1) had the highest rate of survival plantlets in the acclimatization process. Diverse resistance techniques in Onobrychis viciifolia were evaluated when the plants were subjected to water deficiency. Higher frequency of epicuticular waxes was observed in in vivo leaves compared to in vitro leaves. Jagged trichomes nonsecreting glands covered by spines were only observed in the lower leaf side. Ultimately, stomata indices were 0.127 (abaxial), 0.188 (adaxial) in in vivo and 0.121 (abaxial), 0.201 (adaxial) in in vitro leaves.
Related Links	http://dx.doi.org/10.1155/2014/680356
Starting Page	680356
File Format	PDF
ISSN	23566140
e-ISSN	1537744X
Journal	The Scientific World Journal
Volume Number	2014
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2014-01-01
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Environmental Science(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry, Genetics and Molecular Biology Environmental Science

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Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro

In Vitro Micropropagation of Sainfoin ( Onobrychis viciifolia Scop.)

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Micropropagation of Bioencapsulation and Ultrastructural Features of Sainfoin (Onobrychis viciifolia) Grown In Vivo and In Vitro

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