NDLI: Prion Protein and Shadoo Are Involved in Overlapping Embryonic Pathways and Trophoblastic Development

Content Provider	PubMed Central
Author	Bruno, Passet Young, Rachel Samira, Makhzami Marthe, Vilotte Florence, Jaffrezic Sophie, Halliez Bouet, Stéphan Sylvain, Marthey Khalifé, Manal Colette, Kanellopoulos-langevin Vincent, Béringue Provost, Fabienne Le Laude, Hubert Vilotte, Jean-luc
Editor	Mallucci, Giovanna R.
Copyright Year	2012
Abstract	The potential requirement of either the Prion or Shadoo protein for early mouse embryogenesis was recently suggested. However, the current data did not allow to precise the developmental process that was affected in the absence of both proteins and that led to the observed early lethal phenotype. In the present study, using various Prnp transgenic mouse lines and lentiviral vectors expressing shRNAs that target the Shadoo-encoding mRNA, we further demonstrate the specific requirement of at least one of these two PrP-related proteins at early developmental stages. Histological analysis reveals developmental defect of the ectoplacental cone and important hemorrhage surrounding the Prnp-knockout-Sprn-knockdown E7.5 embryos. By restricting the RNA interference to the trophoblastic cell lineages, the observed lethal phenotype could be attributed to the sole role of these proteins in this trophectoderm-derived compartment. RNAseq analysis performed on early embryos of various Prnp and Sprn genotypes indicated that the simultaneous down-regulation of these two proteins affects cell-adhesion and inflammatory pathways as well as the expression of ectoplacental-specific genes. Overall, our data provide biological clues in favor of a crucial and complementary embryonic role of the prion protein family in Eutherians and emphasizes the need to further evaluate its implication in normal and pathological human placenta biology.
Related Links	http://dx.doi.org/10.1371/journal.pone.0041959
Starting Page	41959
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	7
Volume Number	7
Language	English
Publisher	Public Library of Science
Publisher Date	2012-07-30
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation

p27 and Rb are on overlapping pathways suppressing tumorigenesis in mice

Expression of the Prion Protein Family Member Shadoo Causes Drug Hypersensitivity That Is Diminished by the Coexpression of the Wild Type Prion Protein

Conserved Stress-protective Activity between Prion Protein and Shadoo*

Shadoo (Sprn) and prion disease incubation time in mice

Biochemical insight into the prion protein family

The prion protein family member Shadoo induces spontaneous ionic currents in cultured cells

Regulation of sub-compartmental targeting and folding properties of the Prion-like protein Shadoo

Direct observation of multiple misfolding pathways in a single prion protein molecule

Prion Protein and Shadoo Are Involved in Overlapping Embryonic Pathways and Trophoblastic Development

Similar Documents

The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation

p27 and Rb are on overlapping pathways suppressing tumorigenesis in mice

Expression of the Prion Protein Family Member Shadoo Causes Drug Hypersensitivity That Is Diminished by the Coexpression of the Wild Type Prion Protein

Conserved Stress-protective Activity between Prion Protein and Shadoo*

Shadoo (Sprn) and prion disease incubation time in mice

Biochemical insight into the prion protein family

The prion protein family member Shadoo induces spontaneous ionic currents in cultured cells

Regulation of sub-compartmental targeting and folding properties of the Prion-like protein Shadoo

Direct observation of multiple misfolding pathways in a single prion protein molecule

Prion Protein and Shadoo Are Involved in Overlapping Embryonic Pathways and Trophoblastic Development