NDLI: Pharyngeal Polysaccharide Deacetylases Affect Development in the Nematode C. elegans and Deacetylate Chitin In Vitro

Content Provider	PubMed Central
Author	Heustis, Ronald J. Ng, Hong K. Brand, Kenneth J. Rogers, Meredith C. Le, Linda T. Specht, Charles A. Fuhrman, Juliet A.
Editor	Carlow, Clotilde K.
Copyright Year	2012
Abstract	Chitin (β-1,4-linked-N-acetylglucosamine) provides structural integrity to the nematode eggshell and pharyngeal lining. Chitin is synthesized in nematodes, but not in plants and vertebrates, which are often hosts to parasitic roundworms; hence, the chitin metabolism pathway is considered a potential target for selective interventions. Polysaccharide deacetylases (PDAs), including those that convert chitin to chitosan, have been previously demonstrated in protists, fungi and insects. We show that genes encoding PDAs are distributed throughout the phylum Nematoda, with the two paralogs F48E3.8 and C54G7.3 found in C. elegans. We confirm that the genes are somatically expressed and show that RNAi knockdown of these genes retards C. elegans development. Additionally, we show that proteins from the nematode deacetylate chitin in vitro, we quantify the substrate available in vivo as targets of these enzymes, and we show that Eosin Y (which specifically stains chitosan in fungal cells walls) stains the C. elegans pharynx. Our results suggest that one function of PDAs in nematodes may be deacetylation of the chitinous pharyngeal lining.
Related Links	http://dx.doi.org/10.1371/journal.pone.0040426
Starting Page	40426
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	7
Volume Number	7
Language	English
Publisher	Public Library of Science
Publisher Date	2012-07-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Locomotion of C. elegans: A Piecewise-Harmonic Curvature Representation of Nematode Behavior

Description of Caenorhabditis sinica sp. n. (Nematoda: Rhabditidae), a Nematode Species Used in Comparative Biology for C. elegans

Serotonin Control of Thermotaxis Memory Behavior in Nematode Caenorhabditis elegans

Does Malaria Affect Placental Development? Evidence from In Vitro Models

Caenorhabditis elegans: A Simple Nematode Infection Model for Penicillium marneffei

Quantitative Analysis of Cytokinesis In Situ during C. elegans Postembryonic Development

C. elegans ATAD-3 Is Essential for Mitochondrial Activity and Development

Correction: Does Malaria Affect Placental Development? Evidence from In Vitro Models

Correction: Quantitative Analysis of Cytokinesis In Situ during C. elegans Postembryonic Development

Pharyngeal Polysaccharide Deacetylases Affect Development in the Nematode C. elegans and Deacetylate Chitin In Vitro

Similar Documents

Locomotion of C. elegans: A Piecewise-Harmonic Curvature Representation of Nematode Behavior

Description of Caenorhabditis sinica sp. n. (Nematoda: Rhabditidae), a Nematode Species Used in Comparative Biology for C. elegans

Serotonin Control of Thermotaxis Memory Behavior in Nematode Caenorhabditis elegans

Does Malaria Affect Placental Development? Evidence from In Vitro Models

Caenorhabditis elegans: A Simple Nematode Infection Model for Penicillium marneffei

Quantitative Analysis of Cytokinesis In Situ during C. elegans Postembryonic Development

C. elegans ATAD-3 Is Essential for Mitochondrial Activity and Development

Correction: Does Malaria Affect Placental Development? Evidence from In Vitro Models

Correction: Quantitative Analysis of Cytokinesis In Situ during C. elegans Postembryonic Development

Pharyngeal Polysaccharide Deacetylases Affect Development in the Nematode C. elegans and Deacetylate Chitin In Vitro