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| Content Provider | PubMed Central |
|---|---|
| Author | Dean, G. A. Reubel, G. H. Moore, P. F. Pedersen, N. C. |
| Abstract | Feline immunodeficiency virus (FIV) is similar to human immunodeficiency virus type 1 virologically and induces a clinical syndrome in cats comparable to human immunodeficiency virus type 1 syndrome in humans. To determine the lymphoid target cells of FIV, populations of CD4+ lymphocytes, CD8+ lymphocytes, and CD21+ lymphocytes (B cells) were enriched to more than 96.5% purity and then analyzed for FIV provirus by semiquantitative DNA amplification. We found FIV provirus in CD4+, CD8+, and B lymphocytes. In cats infected for <4 months, proviral burden was greatest in CD4+ cells, followed by B cells and then by CD8+ cells. In cats infected for more than 5 years, proviral burden was greatest in B cells, followed by CD4+ cells and then by CD8+ cells. The total proviral burden was > 1 log10 higher in acutely infected cats than in chronically infected cats, primarily because of a higher level of CD4+ infection in the acutely infected cats. A comparison of proviral loads in mesenteric lymph node and peripheral blood mononuclear cells in acutely or chronically infected cats revealed no significant difference. A kinetics study of FIV infection demonstrated that all lymphocyte subpopulations were infected by 4 weeks postinoculation. Virus was isolated from CD4+, CD8+, and B cells in vitro, and reverse transcriptase PCR demonstrated that all subsets contained viral RNA in vivo and therefore are productive reservoirs for FIV. |
| Starting Page | 5165 |
| File Format | |
| ISSN | 10985514 |
| e-ISSN | 10985514 |
| Journal | Journal of Virology |
| Issue Number | 8 |
| Volume Number | 70 |
| Language | English |
| Publisher Date | 1996-08-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Immunology Microbiology Insect Science |
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