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| Content Provider | PubMed Central |
|---|---|
| Author | Eugenia, Gómez-casati María Fuchs, Paul A. Belén, Elgoyhen Ana Katz, Eleonora |
| Copyright Year | 2005 |
| Abstract | Before the onset of hearing, a transient efferent innervation is found on inner hair cells (IHCs). This synapse is inhibitory and mediated by a nicotinic cholinergic receptor (nAChR) probably formed by the α9 and α10 subunits. We analysed the pharmacological and biophysical characteristics of the native nAChR using whole-cell recordings from IHCs in acutely excised apical turns of the rat organ of Corti. Nicotine did not activate but rather blocked the acetylcholine (ACh)-evoked currents with an IC50 of 1 ± 0.1 μm. Antagonists of non-cholinergic receptors such as strychnine, bicuculline and ICS-205930 blocked ACh-evoked responses with an IC50 of 8.6 ± 0.8 nm, 59 ± 4 nm and 0.30 ± 0.02 μm, respectively. The IHC nAChR was both permeable to (P Ca/P Na = 8 ± 0.9) and modulated by external Ca2+. ACh-evoked currents were potentiated by Ca2+ up to 500 μm but were reduced by higher concentrations of this cation. Ba2+ mimicked the effects of Ca2+ whereas Mg2+ only blocked these currents. In addition, elevation of extracellular Ca2+ reduced the amplitude of spontaneous synaptic currents without affecting their time course. The receptor had an EC50 for ACh of 60.7 ± 2.8 μm in 0.5 mm Ca2+. In the absence of Ca2+, the EC50 for ACh increased, suggesting that potentiation by Ca2+ involves changes in the apparent affinity for the agonist. These pharmacological and biophysical characteristics of the IHC nAChR closely resemble those of the recombinant α9α10 nAChR, reinforcing the hypothesis that the functional nAChR at the olivocochlear efferent–IHC synapse is composed of both the α9 and α10 subunits. |
| Related Links | http://dx.doi.org/10.1113/jphysiol.2005.087155 |
| Ending Page | 118 |
| Page Count | 16 |
| Starting Page | 103 |
| File Format | |
| ISSN | 00223751 |
| e-ISSN | 14697793 |
| Journal | The Journal of Physiology |
| Issue Number | Pt 1 |
| Volume Number | 566 |
| Language | English |
| Publisher | Blackwell Science Inc |
| Publisher Date | 2005-07-01 |
| Access Restriction | Open |
| Rights Holder | Blackwell Science Inc |
| Subject Keyword | Physiology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Sports Science |
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