NDLI: SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus

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SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus

Content Provider	MDPI
Author	Matusali, Giulia Colavita, Francesca Lapa, Daniele Meschi, Silvia Bordi, Licia Piselli, Pierluca Gagliardini, Roberta Corpolongo, Angela Nicastri, Emanuele Antinori, Andrea Ippolito, Giuseppe Capobianchi, Maria Rosaria Castilletti, Concetta
Copyright Year	2021
Description	SARS-CoV-2 serum neutralization assay represents the gold standard for assessing antibody-mediated protection in naturally infected and vaccinated individuals. In the present study, 662 serum samples collected from February 2020 to January 2021 from acute and convalescent COVID-19 patients were tested to determine neutralizing antibody (NAb) titers using a microneutralization test (MNT) for live SARS-CoV-2. Moreover, anti-SARS-CoV-2 IgG, IgA, and IgM directed against different viral antigens were measured by high-throughput automated platforms. We observed higher levels of NAbs in elderly (>60 years old) individuals and in patients presenting acute respiratory distress syndrome. SARS-CoV-2 NAbs develop as soon as five days from symptom onset and, despite a decline after the second month, persist for over 11 months, showing variable dynamics. Through correlation and receiver operating characteristic (ROC) curve analysis, we set up a testing algorithm, suitable for the laboratory workload, by establishing an optimal cutoff value of anti-SARS-CoV-2 IgG for convalescent plasma donors to exclude from MNT samples foreseen to have low/negative NAb titers and ineligible for plasma donation. Overall, MNT, although cumbersome and not suitable for routine testing of large sample sizes, remains the reference tool for the assessment of antibody-mediated immunity after SARS-CoV-2 infection. Smart testing algorithms may optimize the laboratory workflow to monitor antibody-mediated protection in COVID-19 patients, plasma donors, and vaccinated individuals.
Starting Page	655
e-ISSN	19994915
DOI	10.3390/v13040655
Journal	Viruses
Issue Number	4
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2021-04-10
Access Restriction	Open
Subject Keyword	Viruses Infectious Diseases Sars-cov-2 Neutralizing Antibodies Protective Immunity Serology
Content Type	Text
Resource Type	Article

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