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Comparison of Four SARS-CoV-2 Neutralization Assays
| Content Provider | MDPI |
|---|---|
| Author | Annika, Rössler Riepler, Lydia Falch, Albert Volland, André Borena, Wegene Laer, Dorothee Von Kimpel, Janine |
| Copyright Year | 2020 |
| Description | Neutralizing antibodies are a major correlate of protection for many viruses including the novel coronavirus SARS-CoV-2. Thus, vaccine candidates should potently induce neutralizing antibodies to render effective protection from infection. A variety of in vitro assays for the detection of SARS-CoV-2 neutralizing antibodies has been described. However, validation of the different assays against each other is important to allow comparison of different studies. Here, we compared four different SARS-CoV-2 neutralization assays using the same set of patient samples. Two assays used replication competent SARS-CoV-2, a focus forming assay and a TCID50-based assay, while the other two assays used replication defective lentiviral or vesicular stomatitis virus (VSV)-based particles pseudotyped with SARS-CoV-2 spike. All assays were robust and produced highly reproducible neutralization titers. Titers of neutralizing antibodies correlated well between the different assays and with the titers of SARS-CoV-2 S-protein binding antibodies detected in an ELISA. Our study showed that commonly used SARS-CoV-2 neutralization assays are robust and that results obtained with different assays are comparable. |
| Starting Page | 13 |
| e-ISSN | 2076393X |
| DOI | 10.3390/vaccines9010013 |
| Journal | Vaccines |
| Issue Number | 1 |
| Volume Number | 9 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2020-12-28 |
| Access Restriction | Open |
| Subject Keyword | Vaccines Virology Sars-cov-2 Neutralizing Antibodies Neutralization Assay Pseudotype Virus |
| Content Type | Text |
| Resource Type | Article |
