NDLI: Dynamic Assay for Profiling Anti-SARS-CoV-2 Antibodies and Their ACE2/Spike RBD Neutralization Capacity

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Dynamic Assay for Profiling Anti-SARS-CoV-2 Antibodies and Their ACE2/Spike RBD Neutralization Capacity

Content Provider	MDPI
Author	Phelan, Thomas Dunne, Jean Conlon, Niall Cheallaigh, Clíona Abbott, W. Faba-Rodriguez, Raquel Amanat, Fatima Krammer, Florian Little, Mark Hughes, Gerry Bergin, Colm Kerr, Colm Sundaresan, Sudharshana Long, Aideen McCormack, William Brady, Gareth
Copyright Year	2021
Description	Serological assays have been widely employed during the coronavirus disease 2019 (COVID-19) pandemic to measure antibody responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to track seroconversion in populations. However, currently available assays do not allow determination of neutralization capacity within the assay protocol. Furthermore, commercial serology assays have a high buy-in cost that is inaccessible for many research groups. We have replicated the serological enzyme-linked immunosorbent assay for the detection of SARS-CoV-2 antibody isotypes, developed at the Icahn School of Medicine at Mount Sinai, New York. Additionally, we have modified the protocol to include a neutralization assay with only a minor modification to this protocol. We used this assay to screen local COVID-19 patient sera (n = 91) and pre-COVID-19 control sera (n = 103), and obtained approximate parity with approved commercial anti-nucleoprotein-based assays with these sera. Furthermore, data from our neutralization assay closely aligns with that generated using a spike-based pseudovirus infection model when a subset of patient sera was analyzed.
Starting Page	1371
e-ISSN	19994915
DOI	10.3390/v13071371
Journal	Viruses
Issue Number	7
Volume Number	13
Language	English
Publisher	MDPI
Publisher Date	2021-07-15
Access Restriction	Open
Subject Keyword	Viruses Infectious Diseases Antibody Enzyme-linked Immunosorbent Assay Neutralization Sars-cov-2 Serology Pseudovirus Infection Model
Content Type	Text
Resource Type	Article

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