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Robust Neutralizing Antibody Levels Detected after Either SARS-CoV-2 Vaccination or One Year after Infection
| Content Provider | MDPI |
|---|---|
| Author | Stefan, Glöckner Bettina, Löffler Hornung, Franziska Baier, Michael Weis, Sebastian Pletz, Mathias W. Deinhardt-Emmer, Stefanie |
| Copyright Year | 2021 |
| Description | Humoral immunity after infection or after vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been attributed a key part in mitigating the further transmission of the virus. In this study, we used a commercial anti-Spike immunoglobulin G (S-IgG) assay and developed a cell culture-based neutralization assay to understand the longitudinal course of neutralizing antibodies in both SARS-CoV2 infected or vaccinated individuals. We show that even more than one year after infection, about 78% of observed study participants remained seropositive concerning S-IgG antibodies. In addition, the serum of the individuals had stable neutralization capacity in a neutralization assay against a SARS-CoV-2 patient isolate from March 2020. We also examined volunteers after either homologous BNT162b2 prime-boost vaccination or heterologous AZD1222 prime/mRNA-based booster vaccination. Both the heterologous and the homologous vaccination regimens induced higher levels of neutralizing antibodies in healthy subjects when compared to subjects after a mild infection, showing the high effectiveness of available vaccines. In addition, we could demonstrate the reliability of S-IgG levels in predicting neutralization capacity, with 94.8% of seropositive samples showing a neutralization titer of ≥10, making it a viable yet cheap and easy-to-determine surrogate parameter for neutralization capacity. |
| Starting Page | 2003 |
| e-ISSN | 19994915 |
| DOI | 10.3390/v13102003 |
| Journal | Viruses |
| Issue Number | 10 |
| Volume Number | 13 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-10-05 |
| Access Restriction | Open |
| Subject Keyword | Viruses Infectious Diseases Sars-cov-2 Covid-19 Vaccination Neutralization Serology Antibodies Immunity |
| Content Type | Text |
| Resource Type | Article |
