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Hyaluronate-Functionalized Graphene for Label-Free Electrochemical Cytosensing
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jing, Aihua Zhang, Chunxin Liang, Gaofeng Feng, Wenpo Tian, Zhengshan Jing, Chenhuan |
| Copyright Year | 2018 |
| Abstract | Electrochemical sensors for early tumor cell detection are currently an important area of research, as this special region directly improves the efficiency of cancer treatment. Functional graphene is a promising alternative for selective recognition and capture of target cancer cells. In our work, an effective cytosensor of hyaluronate-functionalized graphene (HG) was prepared through chemical reduction of graphene oxide. The as-prepared HG nanostructures were characterized with Fourier transform infrared spectroscopy and transmission electron microscopy coupled with cyclic voltammograms and electrochemical impedance spectroscopy, respectively. The self-assembly of HG with ethylene diamine, followed by sodium hyaluronate, enabled the fabrication of a label-free electrochemical impedance spectroscopy cytosensor with high stability and biocompatibility. Finally, the proposed cytosensor exhibited satisfying electrochemical behavior and cell-capture capacity for human colorectal cancer cells HCT-116, and also displayed a wide linear range, from 5.0 × 10² cells∙mL-1 to 5.0 × 10⁶ cells∙mL-1, and a low detection limit of 100 cells∙mL-1 (S/N = 3) for quantification. This work paves the way for graphene applications in electrochemical cytosensing and other bioassays. |
| File Format | PDF HTM / HTML |
| DOI | 10.3390/mi9120669 |
| PubMed reference number | 30567299 |
| Journal | Medline |
| Volume Number | 9 |
| Journal | Micromachines |
| Alternate Webpage(s) | https://res.mdpi.com/micromachines/micromachines-09-00669/article_deploy/micromachines-09-00669.pdf?attachment=1&filename= |
| Alternate Webpage(s) | https://doi.org/10.3390/mi9120669 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
