Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Unsöld, Christine Pappano, William N. Imamura, Yasutada Steiglitz, Barry M. Greenspan, Daniel S. |
| Description | Nonhelical sequence remaining at the carboxyl terminus of a procollagen chain upon cleavage of the C-propeptide (C-telopeptide) Processing intermediate of pro-α1(V) collagen that retains the C-propeptide but from which part of the N-propeptide has been removed by BMP-1 (pCα1(V)) Processed pro-α1(V) chain in which the C-propeptide has been removed and part of the N-propeptide has been removed by BMP-1 (α1(V)) |
| Abstract | The low abundance fibrillar collagen type V is incorporated into and regulates the diameters of type I collagen fibrils. Bone morphogenetic protein-1 (BMP-1) is a metalloprotease that plays key roles in regulating formation of vertebrate extracellular matrix; it cleaves the C-propeptides of the major fibrillar procollagens I–III and processes precursors to produce the mature forms of the cross-linking enzyme prolysyl oxidase, the proteoglycan biglycan, and the basement membrane protein laminin 5. Here we have successfully produced recombinant pro-α1(V)2pro-α2(V) heterotrimers, and we have used these to characterize biosynthetic processing of the most prevalent in vivo form of type V procollagen. In addition, we have compared the processing of endogenous pro-α1(V) chains by wild type mouse embryo fibroblasts and by fibroblasts derived from embryos doubly homozygous null for the Bmp-1 gene and for a gene encoding the closely related metalloprotease mammalian Tolloid-like 1. Together, results presented herein indicate that within pro-α1(V)2pro-α2(V) heterotrimers, pro-α1(V) N-propeptides and pro-α2(V) C-propeptides are processed by BMP-1-like enzymes, and pro-α1(V) C-propeptides are processed by furin-like proprotein convertases in vivo. |
| Related Links | http://www.jbc.org/content/277/7/5596.abstract |
| Ending Page | 5602 |
| Starting Page | 5596 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 7 |
| Volume Number | 277 |
| DOI | 10.1074/jbc.M110003200 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2002-02-15 |
| Access Restriction | Open |
| Subject Keyword | Amino-terminal propeptide (N-propeptide) Carboxyl-terminal propeptide (C-propeptide) Bone morphogenetic protein-1 (BMP-1) Mammalian Tolloid (mTLD) Mammalian Tolloid-like 1 (mTLL-1) Processing intermediate of procollagen chains that contains the N- but not the C-propeptide (pN) Mouse embryo fibroblasts (MEFs) Dulbecco's modified Eagle's medium (DMEM) Phosphate-buffered saline (PBS) Proline- and arginine-rich protein (PARP) GLYCOBIOLOGY AND EXTRACELLULAR MATRICES |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|