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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Tottey, Stephen Rondet, Sabine A. M. Borrelly, Gilles P. M. Robinson, Pamela J. Rich, Peter R. Robinson, Nigel J. |
| Abstract | A bacterial two-hybrid assay revealed interaction between a protein now designated bacterial Atx1 and amino-terminal domains of copper-transporting ATPases CtaA (cellular import) and PacS (thylakoid import) but not the related zinc (ZiaA) or cobalt (CoaT) transporters from the same organism (Synechocystis PCC 6803). The specificity of metallochaperone interactions coincides with metal specificity. After reconstitution in a N2 atmosphere, bacterial Atx1 bound 1 mol of copper mol−1, and apoPacSN acquired copper from copper-Atx1. Copper was displaced from Atx1 byp-(hydroxymercuri)phenylsulfonate, indicative of thiol ligands, and two cysteine residues were obligatory for two-hybrid interaction with PacSN. This organism contains compartments (thylakoids) where the copper proteins plastocyanin and cytochrome oxidase reside. In copper super-supplemented mutants, photooxidation of cytochrome c 6 was greater in Δatx1ΔctaA than in ΔctaA, showing that Atx1 contributes to efficient switching from iron in cytochrome c 6 to copper in plastocyanin for photosynthetic electron transport. Cytochrome oxidase activity was also less in membranes purified from low [copper]-grown Δatx1 or ΔpacS, compared with wild-type, but the double mutant Δatx1ΔpacS was non-additive, consistent with Atx1 acting via PacS. Conversely, activity in Δatx1ΔctaA was less than in either respective single mutant, revealing that Atx1 can function without the major copper importer and consistent with a role in recycling endogenous copper. |
| Related Links | http://www.jbc.org/content/277/7/5490.abstract |
| Ending Page | 5497 |
| Starting Page | 5490 |
| Page Count | 8 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 7 |
| Volume Number | 277 |
| DOI | 10.1074/jbc.M105857200 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2002-02-15 |
| Access Restriction | Open |
| Subject Keyword | Open reading frame (ORF) P-(hydroxymercuri)phenylsulfonate (PMPS) MEMBRANE TRANSPORT STRUCTURE FUNCTION AND BIOGENESIS |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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