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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Caricasole, Andrea Bettini, Ezio Sala, Cinzia Roncarati, Renza Kobayashi, Naoki Caldara, Fabrizio Goto, Kaoru Terstappen, Georg C. |
| Abstract | Diacylglycerol kinases are key modulators of levels of diacylglycerol, a second messenger involved in a variety of cellular responses to extracellular stimuli. A number of diacylglycerol kinases encoded by separate genes are present in mammalian genomes. We have cloned cDNAs encoding several isoforms of the human homologue of the rat diacylglycerol kinase β gene and characterized two such isoforms that differ at their carboxyl terminus through alternative splicing and the usage of different polyadenylation signals. Quantitative analysis of gene expression in a panel of human tissue cDNAs revealed that transcripts corresponding to both isoforms are co-expressed in central nervous system tissues and in the uterus, with one variant being expressed at relatively higher levels. As green fluorescent protein fusions, the two isoforms displayed localization to different subcellular compartments, with one variant being associated with the plasma membrane, while the other isoform was predominantly localized within the cytoplasm. Differences were also observed in their subcellular localization in response to phorbol ester stimulation. Enzymatic assays demonstrated that the two isoforms display comparable diacylglycerol kinase activities. Therefore, the human diacylglycerol kinase β gene can generate several enzyme isoforms, which can display different expression levels and subcellular localization but similar enzymatic activities in vitro. |
| Related Links | http://www.jbc.org/content/277/7/4790.abstract |
| Ending Page | 4796 |
| Starting Page | 4790 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 7 |
| Volume Number | 277 |
| DOI | 10.1074/jbc.M110249200 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2002-02-15 |
| Access Restriction | Open |
| Subject Keyword | Diacylglycerol (DG) Protein kinase C (PKC) Diacylglycerol kinase (DGK) Cysteine-rich domain (CRD) Reverse transcription (RT) Expressed sequence tag (EST) Standard human DGKβ isoform (DGKβ STD) Splice variant 3′ human DGKβ isoform (DGKβ SV3′) Green fluorescence protein (GFP) 12-0-tetradecanoylphorbol-13-acetate (TPA) MECHANISMS OF SIGNAL TRANSDUCTION |
| Alternative Title | Molecular Cloning and Characterization of the Human Diacylglycerol Kinase β (DGKβ) Gene |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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