NDLI: Negative Regulation of the Gene for the Preprothyrotropin-releasing Hormone from the Mouse by Thyroid Hormone Requires Additional Factors in Conjunction with Thyroid Hormone Receptors

Content Provider	Journal of Biological Chemistry (JBC)
Author	Satoh, Teturou Yamada, Masanobu Iwasaki, Toshiharu Mori, Masatomo
Abstract	To gain additional insights into the negative gene regulatory action by triiodothyronine (T3), we isolated a 2-kilobase pair 5′-flanking region of the mouse preprothyrotropin-releasing hormone (ppTRH) gene and characterized the DNA elements mediating inhibitory regulation by T3 in the promoter region. In GH4C1 cells, the expression of the 2-kilobase pair mouse ppTRH 5′-flanking region fused to the luciferase reporter gene occurred by transfection and was significantly suppressed by T3. In contrast, T3 suppression was not observed in T3 receptor (T3R)-deficient CV-1 cells, suggesting that T3Rs were required for the negative regulation. Cotransfected mouse T3R α1, β1, and β2 possessed indistinguishable potency for the negative regulation. Deletion analysis localized the element mediating the negative regulation to the region between −83 and +46, and the sequence downstream of the transcription start site (TSS) between +12 and +46 was found to be essential for the inhibitory regulation. In mobility shift assays, only T3R monomers bound to the element containing a T3 response element half-site at −57. No apparent T3R binding was observed to the element downstream of TSS. Neither the T3 response element half-site nor the element downstream of the TSS confer T3 suppression individually in heterologous promoters. These results indicate that the negative regulation of murine ppTRH gene by T3 might be mediated by the cooperation of T3R monomers with unknown factor(s) interacting with the element downstream of the TSS.
Related Links	http://www.jbc.org/content/271/44/27919.abstract
Ending Page	27926
Starting Page	27919
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	44
Volume Number	271
DOI	10.1074/jbc.271.44.27919
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1996-11-01
Access Restriction	Open
Subject Keyword	Nucleic Acids, Protein Synthesis, and Molecular Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Novel Regulation of Keratin Gene Expression by Thyroid Hormone and Retinoid Receptors

Regulation of the Action of Steroid/Thyroid Hormone Receptors by Medium-chain Fatty Acids

Coordinated Regulation of and Transcriptional Activation by Xenopus Thyroid Hormone and Retinoid X Receptors

Transcription from the Thyroid Hormone-dependent Promoter of the Xenopus laevis Thyroid Hormone Receptor βA Gene Requires a Novel Upstream Element and the Initiator, but Not a TATA Box

Ligand-dependent Antagonism by Retinoid X Receptors of Inhibitory Thyroid Hormone Response Elements

Glucocorticoid Repression of the Mouse Gonadotropin-releasing Hormone Gene Is Mediated by Promoter Elements That Are Recognized by Heteromeric Complexes Containing Glucocorticoid Receptor

A Unique Role of the β-2 Thyroid Hormone Receptor Isoform in Negative Regulation by Thyroid Hormone MAPPING OF A NOVEL AMINO-TERMINAL DOMAIN IMPORTANT FOR LIGAND-INDEPENDENT ACTIVATION

Cell-specific Expression of the Human Gonadotropin-releasing Hormone Gene in Transgenic Animals

Transcriptional Regulation of the Mouse Presenilin-1 Gene

Negative Regulation of the Gene for the Preprothyrotropin-releasing Hormone from the Mouse by Thyroid Hormone Requires Additional Factors in Conjunction with Thyroid Hormone Receptors

Similar Documents

Novel Regulation of Keratin Gene Expression by Thyroid Hormone and Retinoid Receptors

Regulation of the Action of Steroid/Thyroid Hormone Receptors by Medium-chain Fatty Acids

Coordinated Regulation of and Transcriptional Activation by Xenopus Thyroid Hormone and Retinoid X Receptors

Transcription from the Thyroid Hormone-dependent Promoter of the Xenopus laevis Thyroid Hormone Receptor βA Gene Requires a Novel Upstream Element and the Initiator, but Not a TATA Box

Ligand-dependent Antagonism by Retinoid X Receptors of Inhibitory Thyroid Hormone Response Elements

Glucocorticoid Repression of the Mouse Gonadotropin-releasing Hormone Gene Is Mediated by Promoter Elements That Are Recognized by Heteromeric Complexes Containing Glucocorticoid Receptor

A Unique Role of the β-2 Thyroid Hormone Receptor Isoform in Negative Regulation by Thyroid Hormone MAPPING OF A NOVEL AMINO-TERMINAL DOMAIN IMPORTANT FOR LIGAND-INDEPENDENT ACTIVATION

Cell-specific Expression of the Human Gonadotropin-releasing Hormone Gene in Transgenic Animals

Transcriptional Regulation of the Mouse Presenilin-1 Gene

Negative Regulation of the Gene for the Preprothyrotropin-releasing Hormone from the Mouse by Thyroid Hormone Requires Additional Factors in Conjunction with Thyroid Hormone Receptors