NDLI: Purification and cDNA Cloning of Porcine Brain GDP-L-Fuc:N-Acetyl-β-D-Glucosaminide α1→6Fucosyltransferase

Content Provider	Journal of Biological Chemistry (JBC)
Author	Uozumi, Naofumi Yanagidani, Shusaku Miyoshi, Eiji Ihara, Yoshito Sakuma, Takahiko Gao, Cong-Xiao Teshima, Tadashi Fujii, Shigeru Shiba, Tetsuo Taniguchi, Naoyuki
Abstract	GDP-L-Fuc:N-acetyl-β-D-glucosaminide α1→6fucosyltransferase (α1-6FucT; EC 2.4.1.68), which catalyzes the transfer of fucose from GDP-Fuc to N-linked type complex glycopeptides, was purified from a Triton X-100 extract of porcine brain microsomes. The purification procedures included sequential affinity chromatographies on GlcNAcβ1-2Manα1-6(GlcNAcβ1-2Manα1-2)Manβ1-4GlcNAcβ1-4GlcNAc-Asn-Sepharose 4B and synthetic GDP-hexanolamine-Sepharose 4B columns. The enzyme was recovered in a 12% final yield with a 440,000-fold increase in specific activity. SDS-polyacrylamide gel electrophoresis of the purified enzyme gave a major band corresponding to an apparent molecular mass of 58 kDa. The α1-6FucT has 575 amino acids and no putative N-glycosylation sites. The cDNA was cloned in to pSVK3 and was then transiently transfected into COS-1 cells. α1-6FucT activity was found to be high in the transfected cells, as compared with non- or mock-transfected cells. Northern blotting analyses of rat adult tissues showed that α1-6FucT was highly expressed in brain. No sequence homology was found with other previously cloned fucosyltransferases, but the enzyme appears to be a type II transmembrane protein like the other glycosyltransferases.
Related Links	http://www.jbc.org/content/271/44/27810.abstract
Ending Page	27817
Starting Page	27810
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	44
Volume Number	271
DOI	10.1074/jbc.271.44.27810
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1996-11-01
Access Restriction	Open
Subject Keyword	Carbohydrates, Lipids, and Other Natural Products
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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