NDLI: Endoplasmic Reticulum Glucosidase II Is Composed of a Catalytic Subunit, Conserved from Yeast to Mammals, and a Tightly Bound Noncatalytic HDEL-containing Subunit

Content Provider	Journal of Biological Chemistry (JBC)
Author	Trombetta, E. Sergio Simons, Jan Fredrik Helenius, Ari
Abstract	Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of glucosidases I and II in the endoplasmic reticulum (ER). We isolated enzymatically active glucosidase II from rat liver and found that, in contrast with previous reports, it contains two subunits (α and β). Sequence analysis of peptides derived from them allowed us to identify their corresponding human cDNA sequences. The sequence of the α subunit predicted a soluble protein (104 kDa) devoid of known signals for residence in the ER. It showed homology with several other glucosidases but not with glucosidase I. Among the homologues, we identified a Saccharomyces cerevisiae gene, which we showed by gene disruption experiments to be the functional catalytic subunit of glucosidase II. The disrupted yeast strains had no detectable growth defect. The sequence of the β subunit (58 kDa) showed no sequence homology with other known proteins. It encoded a soluble protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. This suggested that the β subunit is responsible for the ER localization of the enzyme.
Related Links	http://www.jbc.org/content/271/44/27509.abstract
Ending Page	27516
Starting Page	27509
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	44
Volume Number	271
DOI	10.1074/jbc.271.44.27509
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1996-11-01
Access Restriction	Open
Subject Keyword	Carbohydrates, Lipids, and Other Natural Products
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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