NDLI: 6′-Sulfo Sialyl Lex but Not 6-Sulfo Sialyl Lex Expressed on the Cell Surface Supports L-selectin-mediated Adhesion

Content Provider	Journal of Biological Chemistry (JBC)
Author	Tsuboi, Shigeru Isogai, Yukihiro Hada, Noriyasu King, Jennifer K. Hindsgaul, Ole Fukuda, Minoru
Abstract	In order to determine if a sulfated oligosaccharide on the cell surface can function as an L-selectin ligand, a novel approach for in vitro transfer of oligosaccharides was utilized (Srivastava, G., Kaun, K. J., Hindsgaul, O., and Palcic, M. M. (1992) J. Biol. Chem. 267, 22356-22361). CHO cells were incubated with synthetic 6′-sulfo sialyl Lex, NeuNAcα2→3(sulfate-6)Galβ1→4(Fucα1→3)GlcNAc or 6-sulfo sialyl Lex, NeuNAcα2→3Galβ1→4[(Fucα1→3)sulfate→6GlcNAc] oligosaccharide linked to C-6 of a fucose residue in GDP-fucose and a milk fucosyltransferase. The resultant CHO cells expressing 6′-sulfo sialyl Lex or 6-sulfo sialyl Lex on their cell surface were tested for adhesion to E-selectin and L-selectin chimeric proteins coated on plates. The results indicate that 6′-sulfo sialyl Lex supports L-selectin-mediated adhesion much better than sialyl Lex similarly tagged on the cell surface. In contrast, 6-sulfo sialyl Lex containing a sulfate group on the N-acetylglucosamine residue did not support adhesion with either selectin. These combined results suggest that 6′-sulfo sialyl Lex is a much better ligand than sialyl Lex oligosaccharide for L-selectin.
Related Links	http://www.jbc.org/content/271/44/27213.abstract
Ending Page	27216
Starting Page	27213
Page Count	4
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	44
Volume Number	271
DOI	10.1074/jbc.271.44.27213
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1996-11-01
Access Restriction	Open
Subject Keyword	Carbohydrates, Lipids, and Other Natural Products
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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