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| Content Provider | IET Digital Library |
|---|---|
| Author | Guzmán, Ana Karina Pérez Ilyina, Anna Cruz, Alejandro Zugasti García, Jose Daniel García Ceniceros, Elda Patricia Segura Hernández, Jose Luis Martínez González, Rodolfo Ramos |
| Abstract | This work focuses on the obtention of magnetic nanoparticles functionalised with heparin (MNPH) and their interaction with Aspergillus niger lipase enzyme. The MNPH were obtained by the coprecipitation method, its characterisation was carried out by Fourier transform infrared spectroscopy, X-ray diffraction, and alternating gradient magnetometry. Functionalisation with heparin was carried out by ultrasound at various times, later the absorption of the enzyme was carried out with two temperatures 4 and 25°C at different concentrations of lipase. For the recovery of the enzyme, a Plackett–Burman design was used, which indicates that the recovered lipase maintains its enzymatic activity when the ionic strength is lower, it was characterised by sodium dodecyl sulphate-page with which it is demonstrated that it is possible to recover the lipase enzyme through the designed nanostructured system. |
| Starting Page | 623 |
| Ending Page | 628 |
| Page Count | 6 |
| Volume Number | 14 |
| e-ISSN | 17500443 |
| Issue Number | Issue 6, May (2019) |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/mnl/14/6 |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/10.1049/mnl.2018.5490 |
| Journal | Micro & Nano Letters |
| Publisher Date | 2019-05-29 |
| Access Restriction | Open |
| Rights Holder | © The Institution of Engineering and Technology |
| Subject Keyword | Alternating Gradient Magnetometry Amorphous And Nanostructured Magnetic Materials Aspergillus Niger Lipase Enzyme Biochemistry Biomedical Material Biotechnology Chitosan–heparin Coprecipitation Method Enzymatic Activity Enzymes Fine-particle Magnetic System Fourier Transform Infrared Spectra Fourier Transform Infrared Spectroscopy Ionic Strength Magnetic NanoParticle Magnetic Particle Magnetic Property of Nanostructure Magnetic Recovery Microorganisms MNPH Molecular Biophysics Nanocomposite Nanofabrication Nanomagnetics Nanomedicine Nanoparticle Nanostructured System Nanotechnology Application in Biomedicine Nanotube And Nanostructured Material Physical Chemistry Physical Chemistry of Biomolecular Solutions And Condensed State Plackett–Burman Design Precipitation Recovered Lipase Sodium Dodecyl Sulphate Structure of Solid Clusters Temperature 25.0 DegC Temperature 4.0 DegC X-Ray Diffraction |
| Content Type | Text |
| Resource Type | Article |
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