NDLI: High-throughput dilution engine for sample preparation on digital microfluidic biochips

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High-throughput dilution engine for sample preparation on digital microfluidic biochips

Content Provider	IET Digital Library
Author	Roy, Sudip Bhattacharya, Bhargab B. Ghoshal, Sarmishtha Chakrabarty, Krishnendu
Abstract	The new generation of digital microfluidic biochips is capable of implementing complex biochemical laboratory assays (bioprotocols) on a tiny device for automatic and reliable analysis of fluid samples. Dilution of a sample fluid is one of the basic steps required in almost all bioprotocols. The authors design a dilution engine for sample preparation that can produce a stream of target droplets with a desired concentration factor by reusing the waste droplets. Also, a scheduling scheme is presented for mapping the dilution steps into the dilution engine. Next, an architecture is proposed to implement the dilution engine using only one (1:1) mix-split microfluidic module (mixer). The authors consider two schemes: (i) the base approach, where no on-chip storage unit (additional electrode) is available, and (ii) when a constant number of storage units is provided. Simulation results show that the second scheme saves the expensive reactants most efficiently, produces a stream of target droplets very fast and is highly suitable for supplying the required number of droplets as needed.
Starting Page	163
Ending Page	171
Page Count	9
ISSN	17518601
Volume Number	8
e-ISSN	1751861X
Issue Number	Issue 4, Jul (2014)
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/iet-cdt/8/4
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/10.1049/iet-cdt.2013.0060
Journal	IET Computers & Digital Techniques
Publisher Date	2013-09-17
Access Restriction	Open
Rights Holder	© The Institution of Engineering and Technology
Subject Keyword	Automatic Analysis Biochemistry Bioprotocols Complex Biochemical Laboratory Assays Concentration Factor Digital Microfluidic Biochips Drops Fluid Sample High-throughput Dilution Engine Lab-on-a-chip Laboratory Technique MEMS And NEMS Device Technology Microfluidics Micromechanical And Nanomechanical Device And System Mix-split Microfluidic Module On-chip Storage Unit Reliable Analysis Sample Fluid Dilution Scheduling Scheme Target Droplets Waste Droplets
Content Type	Text
Resource Type	Article
Subject	Electrical and Electronic Engineering Hardware and Architecture Software

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