NDLI: Selection of aptamers against cardiac troponin I by SELEX

Content Provider	IEEE Xplore Digital Library
Author	Jun Liu Min Li Zhipeng Zeng Ke Song Peidong Xu Juankun Zhang
Copyright Year	2011
Description	Author affiliation: Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology, 300457, China (Jun Liu; Min Li; Zhipeng Zeng; Ke Song; Peidong Xu; Juankun Zhang)
Abstract	The cardiac troponin (cTn) is a regulatory protein related with cardiac contractility variability, it consists of three subunits, cTnI, cTnT and cTnC. Detection of cardiac troponin I in serum is very specific for myocardial injury, cTnI is rapidly released into the serum when the cardiac muscle is damaged. Therefore, cTnI has become the preferred biomarkers of early diagnosis of myocardial injury. The procedure of systematic evolution of ligands by exponential enrichment (SELEX) is a method in which single-stranded oligonucleotides are selected from a wide variety of sequences, based on their interaction with a target molecule. In this work, A 81bp single stranded DNA random library was subjected to 12 rounds of selection against cardiac troponin I by SELEX. The PCR conditions for ssDNA amplification were optimized, for the purpose of establishing a more simple practical SELEX technology to screen the aptamers of cTnI for clinical diagnosis of acute myocardial infarction (AMI). The result showed that, after 12 round selection, the binding rates against cTnI increased from 1.3% at first round to 33.1% at the last round, the specific oligonucleotides were enriched to a considerable degree.
Starting Page	536
Ending Page	539
File Size	202798
Page Count	4
File Format	PDF
ISBN	9781612847016
e-ISBN	9781612847047
DOI	10.1109/ITiME.2011.6130894
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-12-09
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Annealing Microorganisms Laboratories aptamer DNA Myocardium Libraries asymmetric PCR SELEX cardiac troponin I
Content Type	Text
Resource Type	Article

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