NDLI: Full contrast transfer function correction in 3D cryo-EM reconstruction

Content Provider	IEEE Xplore Digital Library
Author	Yi Wan Wah Chiu Zhou, Z.H.
Copyright Year	2004
Description	Author affiliation: Verna & Marrs McLean Dept. of Biochem. & Molecular Biol., Baylor Coll. of Med., Houston, TX, USA (Yi Wan; Wah Chiu)
Abstract	Over the past years electron cryo-microscopy (cryo-EM) has established itself as an important tool in studying the three dimensional structure of biological molecules up to the resolution of 6-9 /spl Aring/. However, as we pursue even higher resolution (i.e., 3-4 /spl Aring/), the depth-of-field problem inherent in the contrast transfer function emerges as a limiting factor. This problem has been previously addressed in the research community (Jensen, G.J., 2000; DeRosier, D.J., 2000; Zhou, Z.H. and Chiu, W., 2003; Cohen, H.A. et al., 1984). We develop a full theoretical solution to this problem. We show that the projected image from the electron microscope corresponds to neither a slice, nor an Ewald sphere, in the Fourier space, but a pair of quadratic surfaces in that space. The general solutions to this problem for both single and double defocus exposures are developed. Simulations show the correctness of the theory.
Sponsorship	Ministry of Educ. (MOE) of PR China City Univ. of Hong Kong K.C. Wong Educ. Found
Starting Page	960
Ending Page	964
File Size	381781
Page Count	5
File Format	PDF
ISBN	0780386477
DOI	10.1109/ICCCAS.2004.1346339
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2004-06-27
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fourier transforms Image resolution Transmission electron microscopy Transfer functions Frequency Educational institutions Biochemistry Electron microscopy Image reconstruction Biomedical imaging
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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