NDLI: Effects on endoplasmic reticulum stress response of applying nanosecond pulsed electric fields

Content Provider	IEEE Xplore Digital Library
Author	Fukuda, H. Miyake, M. Hirai, H. Teranishi, K. Shimomura, N. Oyadomari, S.
Copyright Year	2015
Description	Author affiliation: Tokushima Univ., Tokushima, Japan (Fukuda, H.; Miyake, M.; Hirai, H.; Teranishi, K.; Shimomura, N.; Oyadomari, S.)
Abstract	Recently, pulsed power technology has been applied to biological and medical fields. In particular, nanosecond pulsed electric fields (nsPEFs) have been expected to cause various biological reactions. This study explored to induction of endoplasmic reticulum (ER) stress response by nsPEFs in vivo and in vitro experiments. The ER stress is considered cause of disorders, such as diabetes and obesity. The control of ER stress response by nsPEFs can establish a new prevention and therapy for the disorders. A Blumlien-type pulse forming network (B-PFN) applied 14 ns-width voltage pulses on samples. The phosphorylation of eIF2α (P-eIF2α) was analyzed by western blotting and expression of mRNA related to P-eIF2α were evaluated by RT-PCR analysis. In vivo experiments, skeletal muscles of mice were exposed to the nsPEFs. The amount of Atf3, Atf4, CHOP, and Gadd34 which were ER stress sensor, was evaluated. The nsPEFs application induced the P-eIF2α, but other stress than ER stress response might be included. In vitro experiments adopted cultured cells as HeLa cells, HEK293 cells, and MEF cells. In HeLa cells, the P-eIF2α was confirmed and it depended on the number of applied pulses and the applied electric field. In western blotting for HEK293 and MEF cells, the P-eIF2α might occurred but consistent responses was not confirmed. The sensitivity for the nsPEFs would depend on cell species.
Starting Page	1
Ending Page	4
File Size	610660
Page Count	4
File Format	PDF
e-ISBN	9781479984039
DOI	10.1109/PPC.2015.7296943
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-05-31
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Stress Electric fields Erbium In vivo In vitro Nanobioscience Cells (biology)
Content Type	Text
Resource Type	Article

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