NDLI: Study of effect of pulsing sequence of nanosecond pulsed electric fields on viability of HeLa S3 cell

Content Provider	IEEE Xplore Digital Library
Author	Mitsutake, K. Satoh, A. Mine, S. Abe, K. Katsuki, S. Akiyama, H.
Copyright Year	2010
Description	Author affiliation: Department of New Frontier Sciences, Kumamoto University Kumamoto, Japan (Mitsutake, K.; Satoh, A.; Mine, S.; Abe, K.; Katsuki, S.; Akiyama, H.)
Abstract	Here, we demonstrates the influence of the pulsing sequence of repetitive nanosecond pulsed electric fields on the viability of human cancer cells. A commercially available repetitive magnetic pulse compression generator was used to deliver 70 ns-long high voltage pulses to cultured HeLa cells in a 4 mm-gap cuvette electrode. Two kinds of pulsing sequential patterns, simply repetitive ns pulses and repetitive burst pulses, were used. The repetition frequency is varied from 0.01 to 250 pulses per second (pps), which corresponds to the interval from 100 to 0.004 s, respectively. The electric field and the number of pulses were fixed at 25 kV/cm and 25, respectively, so that the cells were subjected to the sub-lethal condition. Propidium iodide (PI), which fluoresces in red only when being intercalated to DNA, was used to identify dead cells in a population. Statistical analysis of the death ratio in thousands of cells was done with a flow cytometer. The experiment using the simply repetitive pulses shows the cell viability was changed with the repetition frequency even though the electrical dissipated energy in the medium was the same for all conditions. We found that the death ratio was increased with decreasing the pulse repetition frequency down to 0.33 pps, whereas the ratio is decreased with decreasing the frequency below 0.1 pps. Generally speaking, biological processes advance in their own time constant, for instance, the resealing mechanism of nanopores caused by the application of ns pulses lasts for minutes. The dependence of the cell viability on the pulsing sequence might be associated with time constants of biological processes related to the recovery from the field-induced damage.
Starting Page	204
Ending Page	207
File Size	535868
Page Count	4
File Format	PDF
ISBN	9781424471317
ISSN	1930885X
e-ISBN	9781424471324
DOI	10.1109/IPMHVC.2010.5958329
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-05-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	proliferation nanosecond pulsed electric fields (nsPEF) Time frequency analysis pulsing sequence Biomembranes flow cytometry xCELLigence Nanobioscience HeLa S3 cells Electric fields Plasma temperature propidium iodide assay
Content Type	Text
Resource Type	Article

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