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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Kyung-Sun Lee Hui-Bog Noh Mi-Sook Won Yoon-Bo Shim |
| Copyright Year | 2009 |
| Description | Author affiliation: Busan Center, Korea Basic Science Institute, 609-735, S. Korea (Mi-Sook Won) || Department of Chemistry, Pusan National University, Busan, 609-735, S. Korea (Kyung-Sun Lee; Hui-Bog Noh; Yoon-Bo Shim) |
| Abstract | A electrochemical sensor for β-nicotinamide adenine dinucleotide (NADH) has been developed. To fabricate a stable sensor probe, we modified the probe surface with a conjugated polymer (5,2'∶5',2''-terthiophene-3'-carboxylic acid, TTCA), which serves as a base substrate to construct the artificial biomembrane. The AuNPs were electrodeposited onto a carbon electrode (CE) to enhance its electrocatalytic activity, sensitivity, and stability. Cytochrome c (cyt c) and lipid (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, DOPE) were simultaneously immobilized on the poly-TTCA/AuNPs/CE. DOPE and cyt c immobilized onto the poly-TTCA layers were 2.8 × $10^{−9}$ and 1.8 × $10^{−10}$ $mole/cm^{2},$ respectively. To improve the specific biomimetic properties, ubiquinone (UQ) was immobilized onto the modified electrode together. The characteristics of modified electrode were investigated by cyclic voltammetry, impedance spectrometry, differential pulse voltammetry, and QCM. |
| Starting Page | 1784 |
| Ending Page | 1787 |
| File Size | 565648 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781424445486 |
| ISSN | 19300395 |
| DOI | 10.1109/ICSENS.2009.5398453 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2009-10-25 |
| Publisher Place | New Zealand |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Fabrication Biomimetics Biomembranes Polymers Biosensors Electrodes Probes Stability Lipidomics Impedance |
| Content Type | Text |
| Resource Type | Article |
| Subject | Electrical and Electronic Engineering |
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