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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Shi-Wei Lin Chih-Han Chang Che-Hsin Lin |
| Copyright Year | 2009 |
| Description | Author affiliation: Department of Mechanical/Electro-Mechanical Engineering, National Sun Yat-sen University, Kaohsiung, TAIWAN (Che-Hsin Lin) || Institute of Biomedical Engineering, National Cheng Kung University, Tainan, TAIWAN (Shi-Wei Lin; Chih-Han Chang) |
| Abstract | This paper presents an advanced diascopic illumination technique for simultaneously multi-wavelength fluorescence excitation and detection without any filter sets. High-throughput capillary electrophoresis detection can be realized with this simple and novel approach. The developed system is composed of a home-built dark-field condenser for fluorescence excitation and an ultraviolet-visible-near infrared (UV-VIS-NIR) spectrometer for emitted signal detection. Experimental results show that the proposed system can effectively increase the fluorescent signal. In CE experiment, a fluorescent sample, Rhodamine B, can be successfully detected using this proposed system. Furthermore, a mixed bio-sample composed of three single-strand DNAs labeled with Cy3, FITC, and Alexa647 are successfully separated and detected in a single test run. Results indicate the developed system is capable of achieving full wavelength-resolved fluorescence detection in a single microfluidic channel. The measured detection limit for the developed system is up to 5×10−8} M (S/N = 3) while detecting a standard fluorescein, which is suitable for general bio-analytical applications. |
| Starting Page | 928 |
| Ending Page | 931 |
| File Size | 991477 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781424445486 |
| ISSN | 19300395 |
| DOI | 10.1109/ICSENS.2009.5398174 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2009-10-25 |
| Publisher Place | New Zealand |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Lighting Fluorescence Electrokinetics Infrared spectra Filters Infrared detectors Spectroscopy Signal detection Testing Microfluidics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Electrical and Electronic Engineering |
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