NDLI: Monodisperse Lipoplex Generation by Integrated Picoliter Micro Reactor and Incubator

Content Provider	IEEE Xplore Digital Library
Author	Hsieh, A.T.-H. Lin, A.Y. Lee, A.P.
Copyright Year	2006
Description	Author affiliation: Div. of Biomed. Eng., California Univ., Irvine, CA (Hsieh, A.T.-H.; Lin, A.Y.; Lee, A.P.)
Abstract	A non-viral and efficient carrier for the applications delivering drugs, plasmic DNA, shRNA for pharmaceutical and therapeutic applications is presented. The cationic lipid based liposomal carriers which has been the most attractive non-viral solution. The size of complexes of cationic lipid and DNA (lipoplex) dictates the efficiency of gene transfection. Moreover, the order of mixing of cationic lipid and DNA, mixing rate, and mixture incubation time are factors that determine the lipoplex sizes during sample preparation. We developed a Picoliter Micro Reactor and Incubator (PMRI) system to formulate monodisperse cationic lipids and DNA complexes. The monodisperse micro reactor and incubator was designed and fabricated based on the microfluidic droplet formation devices which developed by our lab and others. Compared with the conventional irreproducible lipoplex preparation using hand-shaking or vortexing processes for mixing, the PMRI system we developed for lipoplex formulation demonstrates the ability of rapid and uniformly mix cationic lipid and DNA simultaneously, and generates a narrower size distribution peak and smaller average size. The PMRI also enables the mixing and incubation integrated process and lipolex formulation reproducibility
Starting Page	158
Ending Page	161
File Size	707604
Page Count	4
File Format	PDF
ISBN	1424403375
DOI	10.1109/MMB.2006.251516
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-05-09
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Inductors DNA Lipidomics Microfluidics Drugs Pharmaceuticals Magnetic resonance imaging Reproducibility of results Petroleum Biology gene transfection micro-droplet micro-reactor micro-incubator lipoplex
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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