NDLI: Characterization of the Membrane Transport Assay System Using Microchamber Array

Content Provider	IEEE Xplore Digital Library
Author	Suzuki, H. Tabata, K.V. Noji, H. Takeuchi, S.
Copyright Year	2006
Description	Author affiliation: Inst. of Ind. Sci., Tokyo Univ. (Suzuki, H.)
Abstract	We have been developing a measurement system for membrane transport across a reconstituted planar lipid bilayer to examine the functions of transporter membrane proteins using a microchamber array (membrane microchamber system). In this system, an artificial planar lipid bilayer is pressed on the parylene microchamber array (typically 0.1~1 pL in volume) fabricated on a coverglass to enclose the volume. After membrane proteins are reconstituted in the bilayer, fluorescently labeled molecules transported across the bilayer are accumulated in microchambers, and detected by fluorescent imaging. The microchamber array is compatible with high-sensitive biological imaging techniques, such as a confocal microscopy and a total internal reflection fluorescence microscopy (TIRFM). Due to the tiny volume of microchambers, concentration of the transported molecules rapidly increases. In this study, the detection system was characterized in detail. With the aid of microchambers, the concentration reached to the detectable level with as small as $10^{2}~10^{3}$ fluorescent molecules
Starting Page	301
Ending Page	302
File Size	2378369
Page Count	2
File Format	PDF
ISBN	1424403375
DOI	10.1109/MMB.2006.251556
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-05-09
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Biomembranes Fluorescence Lipidomics Proteins Microscopy Plasma applications Plasma transport processes Molecular biophysics Optical imaging Polymer films Lipid bilayer Membrane transport Micro chambers
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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