NDLI: Cell migration assay using multiple laminar flows in PDMS microchannel

Content Provider	IEEE Xplore Digital Library
Author	Fu-Qiang Nie Kobayashi, J. Yamada, M. Yamato, M. Kikuchi, A. Okano, T.
Copyright Year	2007
Description	Author affiliation: Tokyo Women's Med. Univ., Tokyo (Fu-Qiang Nie; Kobayashi, J.; Yamada, M.; Yamato, M.; Kikuchi, A.; Okano, T.)
Abstract	Cell migration is a cellular process that plays a critical role in health and disease, including embryogenesis, wound healing, immune response, and tissue development. In order to overcome some shortcomings derived from traditional physical scrape-based cell migration assay, multiple laminar flows have been used to partially treat a confluent cell sheet formed in microchannels, which can simply pattern physiological wound edge. In the present study, rapid prototyping of poly(dimethylsiloxane) (PDMS) microchannels for assays was preformed utilizing instead a simple photopolymerization procedure adapted for a conventional liquid crystal display projector (LCDP) without any need for expensive photomasks. PDMS microchannels having three inlets (300 mum in width) that converged into a single main channel (900 mum in width) were fabricated with the LCDP-modified device. In conclusion, wound edges of confluent cell monolayers for cell migration assay were prepared utilizing trypsin laminar flow to contact only a portion of cell monolayers, within microfluidic channels. These results demonstrate that cell migration assays could be performed even in a miniaturized platform. The utilization of miniaturized microchannels can minimize the amount of expensive reagents required for cell migration assay and allows for possible scaling-up in future applications.
Starting Page	406
Ending Page	409
File Size	1795577
Page Count	4
File Format	PDF
ISBN	9781424418572
DOI	10.1109/MHS.2007.4420889
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-11-11
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microchannel Wounds Microfluidics Cells (biology) Reservoirs Liquid crystal displays Bovine Microscopy Biomedical engineering Diseases
Content Type	Text
Resource Type	Article

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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