NDLI: In-situ Single Cell Mechanics Characterization of W303 Yeast Cells using Nanoneedles inside Environmental-SEM

Content Provider	IEEE Xplore Digital Library
Author	Ahmad, M.R. Nakajima, M. Kojima, S. Homma, M. Fukuda, T.
Copyright Year	2007
Description	Author affiliation: Nagoya Univ., Nagoya (Ahmad, M.R.; Nakajima, M.)
Abstract	In this paper, the characterizations of cellular mechanics of W303 yeast cells have been conducted using nanoneedles inside environmental-SEM (ESEM). This enhanced ESEM system comprises of a standard ESEM instrument as a nano imaging tool, a cooling stage as a cellular biology's humidity controller and 7 D.O.F. linear actuators as nanomanipulator/effector. Four types of nanoneedles have been used in the experiments, i.e. silicon (Si) nanoneedle, titanium (Ti) coated Si nanoneedle and tungsten (W) nanoneedles. The former two nanoneedles are fabricated using 2 N/m spring constant's cantilevers. While the latter nanoneedles are fabricated using 0.09 N/m and 2 N/m spring constant's cantilevers $(W_{0.09}$ and $W_{2}$ nanoneedles). All types of the nanoneedles, i.e. Si, Ti-Si, $W_{0.09}$ and $W_{2}$ nanoneedles are able to be used for single cells' local stiffness characterizations. This ability can be used in future fast disease detection where the disease cells may shows different cell mechanics properties compare to the normal cell. In addition to this, the Si-Ti and $W_{2}$ nanoneedles have shown an ability to penetrate the cell without cell bursting. This ability is important in the future single cell surgery where the cell damage can be eliminated as much as possible.
Starting Page	1
Ending Page	6
File Size	4144144
Page Count	6
File Format	PDF
ISBN	9781424418572
DOI	10.1109/MHS.2007.4420816
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-11-11
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fungi Nanobioscience Springs Diseases Instruments Cooling Humidity control Temperature control Biological control systems Control systems nanoneedles Environmental-SEM nanomanipulation single cell analysis
Content Type	Text
Resource Type	Article

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In Situ Single Cell Mechanics Characterization of Yeast Cells Using Nanoneedles Inside Environmental SEM

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