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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Wang, Z. El-Ali, J. Perch-Nielsen, I.R. Mogensen, K.B. Snakenborg, D. Kutter, J.P. Wolff, A. |
| Copyright Year | 2004 |
| Description | Author affiliation: MIC, Tech. Univ. Denmark, Lyngby, Denmark (Wang, Z.; El-Ali, J.; Perch-Nielsen, I.R.; Mogensen, K.B.; Snakenborg, D.; Kutter, J.P.; Wolff, A.) |
| Abstract | Flow cytometry is a very powerful method for biophysical measurement of microparticles, such as cells and bacteria. In this paper, we report an innovative microsystem, in which several different optical elements (waveguides, lenses and fiber-to-waveguide couplers) are integrated with microfluidic channels to form a complete microchip flow cytometer. All the optical elements, the microfluidic system, and the fiber-to-waveguide couplers were defined in one layer of polymer (SU-8, negative photoresist) by standard photolithography. With only one single mask procedure, all the fabrication and packaging processes can be finished in one day. Polystyrene beads were measured in the microchip flow cytometer, and three signals (forward scattering, large angle scattering and extinction) were measured simultaneously for each bead. The average intensities of the forward Scattered light and the incident light extinction showed excellent linear relationship with the sizes of the beads. To our knowledge this is the first time forward scattered light and incident light extinction were measured in a microsystem using integrated optics. The microsystem can be applied for analyzing different kinds of particles and cells, and can easily be integrated with other microfluidic components. |
| Sponsorship | IEEE Robotics and Automation Soc |
| Starting Page | 367 |
| Ending Page | 370 |
| File Size | 277759 |
| Page Count | 4 |
| File Format | |
| ISBN | 078038265X |
| DOI | 10.1109/MEMS.2004.1290598 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2004-01-25 |
| Publisher Place | Netherlands |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Optical polymers Optical scattering Image motion analysis Integrated optics Fluid flow measurement Light scattering Particle scattering Optical fiber couplers Biomedical optical imaging Microfluidics |
| Content Type | Text |
| Resource Type | Article |
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