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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Jun Xie Jason Shih Changlin Pang Yu-Chong Tai Yunan Miao Lee, T.D. |
| Copyright Year | 2004 |
| Description | Author affiliation: Caltech Micromachining Lab., California Inst. of Technol., Pasadena, CA, USA (Jun Xie; Jason Shih; Changlin Pang; Yu-Chong Tai) |
| Abstract | This work reports the first on-chip generation of gradient elution using electrochemical pumping, for application in Liquid Chromatography-Electrospray Ionization (LC-ESI). The system consists of Parylene, SU-8 and PDMS parts to create two 3 /spl mu/L on-chip solvent reservoirs, two electrochemical pumps and an ESI nozzle. Pumping is controlled galvanostatically (i.e., through electrolysis current). At low back pressures, pumping rate can reach 200 nL/min using just 40 /spl mu/A (power consumption <100 /spl mu/W). At 80 psi, 200 /spl mu/A (power consumption <1 mW) is sufficient to deliver fluid at 75 nL/min. Higher electrical currents have been used to increase the flow rate or to achieve pumping at higher back pressures. For example, pumping at 200 psi back pressure has been demonstrated using a current of 800 /spl mu/A. Electrically controlled on-chip gradient generation is achieved without any external fluidic connections to the chip. Continuous generation of gradients for 30 minutes at a total flow rate of /spl sim/100 nL/min is also realized and confirmed with mass spectroscopy (MS) measurements. The developed on-chip gradient generation system meets the general requirements of LC applications. |
| Sponsorship | IEEE Robotics and Automation Soc |
| Starting Page | 334 |
| Ending Page | 337 |
| File Size | 304078 |
| Page Count | 4 |
| File Format | |
| ISBN | 078038265X |
| DOI | 10.1109/MEMS.2004.1290590 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2004-01-25 |
| Publisher Place | Netherlands |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Pumps System-on-a-chip Energy consumption Ionization Solvents Reservoirs Electrochemical processes Fluid flow control Mass spectroscopy Fluid flow measurement |
| Content Type | Text |
| Resource Type | Article |
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