NDLI: Development of high voltage pulse inducement method for biological cell

Content Provider	IEEE Xplore Digital Library
Author	Adon, M.N. Dalimin, M.N. Jamil, M.M.A. Kassim, N.M.
Copyright Year	2012
Description	Author affiliation: Centre of Optoelectronic Technology, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, Malaysia (Kassim, N.M.) \|\| Department of Electronic Engineering, Modeling and Simulation Research Laboratory, Faculty of Electrical and Electronics Engineering, Malaysia (Adon, M.N.; Jamil, M.M.A.) \|\| Department of Physics, Faculty of Science, Technology and Human Development, Universiti Tun Hussein Onn Malaysia, Malaysia (Dalimin, M.N.)
Abstract	Electroporation (EP) system is a process of controlling cell functions by using electromagnetic fields (EMF) to create pores through a cellular membrane that causes cell lysis and apoptosis. In this paper we present an experimental setup for fundamental studies on cell EP. An adjustable high voltage pulse generator (3kV/10µs – 600µs pulse length) system were connected to the EP chamber which subsequently allow real time observation of membrane permeability changes and cellular physiology. In order to initiate higher cell viability rate, high transfection efficiency, lower sample contamination and smaller Joule heating the modification of EP chamber need to be implemented‥ Following that, HeLa cell culture has been projected as cell that will be used in this study. Finally, some suggestions are proposed for the future studies.
Starting Page	501
Ending Page	503
File Size	1533536
Page Count	3
File Format	PDF
ISBN	9781457719905
e-ISBN	9781457719912
DOI	10.1109/ICoBE.2012.6179068
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-02-27
Publisher Place	Malaysia
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Real time systems Real time monitoring Microscopy Electroporation Biomembranes Biology Electromagnetic field Temperature control Electric fields High voltage pulse Biomedical engineering
Content Type	Text
Resource Type	Article

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