NDLI: Cloning and sequence analysis of a partial L-lactate dehydrogenase gene of Bacillus coagulans Tl-4v

Content Provider	IEEE Xplore Digital Library
Author	Binzhe Wang Qiang Gao Bing Liu Kunyu Jiang Zhe Su
Copyright Year	2010
Description	Author affiliation: School of Biotechnology, Tianjin University of Science and Technology, 300457, China (Binzhe Wang; Qiang Gao; Bing Liu) \|\| Tianjin Key Laboratory of Industrial Microbiology, 300457, China (Kunyu Jiang) \|\| Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin 300457, China (Zhe Su)
Abstract	Bacillus coagulans TL-4 strain is a thermophilic bacterium for high L-lactate production. We reported here that its 779-bp partial $NAD^{+}-dependent$ L-lactate dehydrogenase (LDH) gene sequence (GenBank accession no. GU354320) encoding a deduced 259 amino acid peptide was cloned by using one pair of designed degenerate primers. The amino acid sequence alignment and the built phylogenetic tree revealed that this partial LDH of B. coagulans TL-4 shared high homology with the conserved LDH stretches from 59.17% to 74.70% among other 10 Bacillus species and 48.81% to that of Lactobacillus sp. MD-1. Furthermore, three highly conservative LDH regions, one $(Val1∼Asp^{34})$ for NADH binding site and two $(Asp^{59}∼Ile^{86}$ and $Asn^{107}∼Arg^{139})$ for the distinct active sites, were also found in this $NAD^{+}-dependent$ LDH stretch.
Starting Page	341
Ending Page	344
File Size	264840
Page Count	4
File Format	PDF
ISBN	9781424467754
e-ISBN	9781424467754
DOI	10.1109/ICBBT.2010.5478944
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-04-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Biotechnology Sequences Lactate dehydrogenase (LDH) Laboratories Cloning Genomics B. coagulans Degenerate primers Partial gene cloning Amino acids Capacitive sensors Biochemistry Sequence homology DNA Bioinformatics
Content Type	Text
Resource Type	Article

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