NDLI: Polysaccharides extraction from submerged-cultured mycelium of Grifola frondosa

Content Provider	IEEE Xplore Digital Library
Author	Gao-Qiang Liu Wen-Jun Han Huai-Yun Zhang Xian-Chun Jin
Copyright Year	2010
Description	Author affiliation: Core Facilities of Biotechnology, Central South University of Forestry & Technology, Changsha 410004, China (Gao-Qiang Liu; Wen-Jun Han; Huai-Yun Zhang) \|\| College of Life Science, Henan Agriculture University, Zhengzhou 450002, China (Xian-Chun Jin)
Abstract	Alkaline (NaOH) hydrolysis and aqueous extraction method were comparatively studied in polysaccharides extraction from mycelia of Grifola frondosa obtained with submerged-culture. The results show that the polysaccharide content and the total extract rate from alkaline extraction method were obviously higher than those from the aqueous extraction method. The conditions of alkaline extraction were further optimized by the orthogonal matrix method, and the results reveal that the optimum extraction conditions were as follows: ratio of alkaline volume to mycelium weight was 15 (mL/g), alkaline concentration was 1.0 mol/L, hydrolysis temperature was 70°C, and hydrolysis time was 1.5 hour. With the optimized condition, the polysaccharide extraction rate reached 11.18 ± 0.37 %. The mycelial polysaccharide obtained with alkaline hydrolysis method was fractionated by the DEAE column chromatography. Four G. frondosa polysaccharide (GFPS) fractions were isolated in the polysaccharide sample with GFPS-1 as the main component.
Starting Page	111
Ending Page	114
File Size	185656
Page Count	4
File Format	PDF
ISBN	9781424467754
e-ISBN	9781424467754
DOI	10.1109/ICBBT.2010.5478998
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-04-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Submerged fermentation Temperature Optimization methods Capacitive sensors Extraction technology Neoplasms Fungi Microorganisms Cells (biology) Forestry Grifola frondosa Biomembranes Biochemical engineering Polysaccharide Immune system
Content Type	Text
Resource Type	Article

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