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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Li Xingfeng Wang Zhixin Dong Xinying Wang Guangxian Jia Yingmin |
| Copyright Year | 2011 |
| Description | Author affiliation: College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, China (Li Xingfeng; Wang Zhixin; Dong Xinying; Wang Guangxian; Jia Yingmin) |
| Abstract | Recently, antimicrobial peptides (AMP) produced by bacteria, called bacteriocins, have attracted a great deal of attention as biopreservatives in the application of agri-food industry. Extensive industrial uses and research studies require an accurate and precise method for the quantification of novel AMP in food samples, fermentation broth and other media. In this work, an agar diffusion bioassay was developed for the rapid, accurate and precise quantification of a novel AMP produced by Brevibacillus laterosporus. The pre-diffusion step resulted in larger inhibition zones and more precise results, and the best combination was the method using Staphyloccocus aureus as an indicator strain with pre-diffusion at 4 °C for 12 h. To determine the activity units per millilitre of unknown bacteriocin (AU/ml), serial dilutions of the filter-sterilized supernatant were performed. The relationship between the bacteriocin concentration and the resulting zone of inhibition was demostrated and a high correlation coefficient (R2=0.9886) was obtained. This method may be also applied for the quantification of unknown bacteriocin. |
| Starting Page | 1096 |
| Ending Page | 1099 |
| File Size | 124199 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781424495740 |
| e-ISBN | 9781424495771 |
| DOI | 10.1109/ICAE.2011.5943979 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2011-05-27 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Gold Microorganisms Purification Pre-diffusion Peptides Agar diffusion bioassay Anti-bacterial Potency Equations Antimicrobial peptides (AMP) Immune system |
| Content Type | Text |
| Resource Type | Article |
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