NDLI: Separation and analysis of EGCG from crude tea polyphenols by high-speed countercurrent chromatography

Content Provider	IEEE Xplore Digital Library
Author	Huawei Zhang Junqing Qian
Copyright Year	2011
Description	Author affiliation: School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China (Huawei Zhang; Junqing Qian)
Abstract	In order to establish a novel technique of separation and analysis of EGCG from tea polyphenols using high-speed countercurrent chromatography (HSCCC), a set of HSCCC apparatus was applied to isolate and purify EGCG from tea polyphenols using two-phase solvent system composed of n-hexane-ethyl acetate-ethnol-water-acetic acid (1:5:1:5:0.25, v:v) in tail to head elution model, which the upper phase was stationary and the lower was mobile. The preferable conditions for HSCCC separation of EGCG were as follows: temperature 15 °C, rotation speed 800 r/min, flow-rate 2.0 mL/min, UV detection wavelength 280 nm. The purity of EGCG was analyzed by HPLC and its structure was confirmed by ESI-MS and 1H NMR. The result suggested that EGCG with a purity of 97.5% was successively isolated and undoubtedly identified by 1H NMR and ESI-MS, which yield was 34%. HSCCC was a facile and powerful technique to separate EGCG from tea polypheols.
Starting Page	3516
Ending Page	3519
File Size	580809
Page Count	4
File Format	PDF
ISBN	9781612844589
e-ISBN	9781612844596
DOI	10.1109/CECNET.2011.5768421
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-04-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Purification Separation Humans EGCG Tea polyphenols Nuclear magnetic resonance Colon High-speed countercurrent chromatography Compounds Research and development Cancer
Content Type	Text
Resource Type	Article

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