NDLI: Community Diversity of Nitrosobacteria in SBR Denitrified System for Treating the Livestock Wastewater

Content Provider	IEEE Xplore Digital Library
Author	Xie Qing Dan Dezhong Wu Yang Deng Shihuai
Copyright Year	2011
Abstract	Short-cut nitrification and denitrification had achieved in this Sequencing Batch Reactor (SBR). At the aerating stage, nitrosobacteria had survived much better than nitrobacteria and the concentration ratio of [NO2--N]/[NO3--N]was exceed 50%. After one cycle running, the total ammonia was removed completely. We wished to discover if we could gain greater insights into the relationship between the community construction of nitrosobacteria and the accumulation of nitrite nitrogen in this reactor. This examination used the molecular biology technology by amplifying the target fragment of amoA gene from total DNA of activated sludge, by coupling the target gene with pGM-Tvector and transforming them into E.coli DH5a competent cells. Randomly, picked some white clones out from the LBsolid nutritive medium, and used RFLP markers to obtain the restriction patterns. RFLP analysis showed that there were 8kinds of restriction patterns among these clones. After comparing homologies with standard strains, the results confirmed that there were 3 genera of nitrosobacteria in this reactor: Nitrosospira sp., Nitrosomomas sp. and Nitrosococcussp., the distributing frequencies were 0.285, 0.392 and 0.035, respectively. Preliminary analysis, Nitrosolobus multiform sand Nitrosomomas europaea had played the predominant role in accumulating nitrite nitrogen.
Starting Page	690
Ending Page	693
File Size	267232
Page Count	4
File Format	PDF
ISBN	9781612842783
DOI	10.1109/CDCIEM.2011.484
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-02-19
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Restriction fragment length polymorphisms(RFLP) Microorganisms Communities Sequencing Batch Reactor (SBR) DNA Nitrosobacteria Community diversity Nitrogen Wastewater Inductors
Content Type	Text
Resource Type	Article

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