NDLI: Rapid Multiplexed Imaging of Cell-Surface Cancer Biomarkers in Fresh Tissues with Targeted SERS Nanoparticles

Content Provider	IEEE Xplore Digital Library
Author	Wang, Y.W. Khan, A. Leigh, S.Y. Liu, J.T.C.
Copyright Year	2014
Description	Author affiliation: Dept. of Mech. Eng., Univ. of Washington, Seattle, WA, USA (Wang, Y.W.; Khan, A.; Leigh, S.Y.; Liu, J.T.C.)
Abstract	Our lab is developing miniature Raman imaging systems and topical-staining protocols to rapidly image cell-surface biomarkers in fresh tissues. In particular, this work employs targeted surface-enhanced Raman scattering (SERS) nanoparticles (NPs) to enable the sensitive and multiplexed detection of a large number of cell-surface biomarkers of cancer. The SERS NPs were functionalized with different targeting antibodies, and their biomarker detection capability was investigated via in vitro and ex vivo experiments with cells and tissues. Here, we design SERS NPs to specifically target the cancer biomarker EGFR upon topical application on cells and tissues. In vitro flow cytometry with fluorescent SERS NPs reveals a high ratio of specific versus nonspecific binding for the tumor cell lines A431 (skin cancer), U251 (glioma) and SkBr3 (breast cancer). For tissue imaging, we have developed a fiber-optic-based spectral detection probe, with 785-nm laser illumination, for rapid detection of SERS NPs with sub-millimeter spatial resolution. Based on the spectral detection probe, multiple imaging systems were customized for rapid tissue phenotyping such as a comprehensive rotational scanning endoscope for in vivo imaging of the rat esophagus and a raster-scanning device for intraoperative imaging of breast tissue margins. Ex vivo experiments were performed to develop a strategy for the rapid detection of multiple cell-surface biomarkers following a brief (5-10 min) topical application of SERS NPs on tissues. By developing high-affinity targeted SERS NPs, sensitive spectral-imaging devices, and an optimized topical-delivery protocol, we demonstrate a ratio metric method to rapidly quantify the specific binding of biomarker-targeted NPs on fresh tissues, thereby eliminating the ambiguities that often arise due to nonspecific sources of contrast. These tools will enable multiplexed molecular imaging for the early detection of epithelial cancers, rapid surgical guidance, and monitoring the molecular response to treatments.
Starting Page	149
Ending Page	152
File Size	3391112
Page Count	4
File Format	PDF
ISBN	9781467367523
DOI	10.1109/ISOT.2014.44
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-11-05
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Multiplexing Nanoparticles Cancer biomarkers Imaging Biomarkers SERS nanotags Multiplexed molecular imaging Raman spectroscopy Probes Early detection Tumors Esophagus
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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