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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Li Xiaojuan Chen Cunshe Lv Huitian |
| Copyright Year | 2007 |
| Description | Author affiliation: Capital Normal Univ., Beijing (Li Xiaojuan) |
| Abstract | A biosensor building on $F_{0}F_{1}-ATPase$ for clenbuterol detection is constructed. The energy for ATP synthesis is provided from downhill proton transport along the proton gradient across membranes. This potential is formed by the electron transfer chains of respiration. The fluorescence probe F1300, which was sensitive to pH changes, was labeled into inner chromatophores to indicate the proton-flux during ATP synthesis. It's found that different loads onto the beta-subunit of chromatophores could affect its synthesis activity with different level. According to this principle, a biosensor was constructed based on ATPase to detect clenbuterol and the detecting mechanism depended on proton-flux driven by rotary catalytic ATP synthesis. The experiment results showed that the precision of clenbuterol detection by the biosensor could reach $10^{-12}$ g/L and the new biosensor might prove to be a useful nano-device for super-sensitive detection. |
| Starting Page | 94 |
| Ending Page | 97 |
| File Size | 2494886 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781424408177 |
| DOI | 10.1109/ICCA.2007.4376325 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2007-05-30 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Biosensors Fluorescence Probes Protons Micromotors Chemical technology USA Councils Sugar Biomembranes Biochemistry clenbuterol biosenso FoF |
| Content Type | Text |
| Resource Type | Article |
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