Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Huh, S. Eom, S. Ker, D.F.E. Weiss, L. Kanade, T. |
| Copyright Year | 2012 |
| Description | Author affiliation: Robotics Institute, Carnegie Mellon University (Huh, S.; Eom, S.; Weiss, L.; Kanade, T.) || Department of Biological Sciences and Biomedical Engineering, Carnegie Mellon University (Ker, D.F.E.) |
| Abstract | Understanding the heterogeneous behavior of hematopoietic stem cells (HSCs) is required for the expansion of the cells without loss of their regenerative capacity. As such, it is essential to establish their lineage relationships by tracking the history of individual cells in a cell population. However, the quality of lineage relationships is often degraded because of undetected or misdetected mitotic events, which lead to missed or inaccurate mother-daughter cell relationships. In this paper, we present an automated mitosis detection method for HSCs in time-lapse phase-contrast microscopy images. Since HSCs are nonadherent, i.e., free-floating in the culture medium, the method is distinguished from the recent mitosis detection methods developed for adherent cells that are attached to the surface of a petri dish. The proposed mitosis detection method detects individual cells in each image frame and subsequently tracks them over time and in so doing identifies newly appeared cells, each of which is considered as a candidate of a newborn cell. Each candidate is then examined to determine whether it is indeed a newborn cell based on temporal change of cell sizes of potential mother and daughter cells. Our method was quantitatively evaluated on 14 HSC populations, each of which is observed for four days, resulting in a precision of 97.4% and a recall of 96.6%. |
| Starting Page | 390 |
| Ending Page | 393 |
| File Size | 499815 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781457718571 |
| ISSN | 19457928 |
| e-ISBN | 9781457718588 |
| DOI | 10.1109/ISBI.2012.6235566 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2012-05-02 |
| Publisher Place | Spain |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Stem cells Pediatrics Microscopy Feature extraction Probabilistic logic Cells (biology) Blood microscopy time-lapse image analysis hematopoietic stem cells (HSC) mitosis detection cell tracking probabilistic temporal modeling |
| Content Type | Text |
| Resource Type | Article |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|