NDLI: Insulin stimulates monocyte membrane associated tissue factor activity

Content Provider	IEEE Xplore Digital Library
Author	Bhatt, B.A. Patel, D.R. Kristol, D. Arora, R.R. Spillert, C.R.
Copyright Year	2003
Description	Author affiliation: New Jersey Inst. of Technol., Newark, NJ, USA (Bhatt, B.A.; Patel, D.R.; Kristol, D.)
Abstract	The benefits of intensive insulin therapy for type 2 diabetics with cardiovascular disease (CVD) are highly controversial. We performed in vitro studies to determine whether insulin has any effect on whole blood coagulation and assess how insulin responds to monocyte membrane associated tissue factor (mmaTF) induced hypercoagulability. Mercuric ion (Hg/sup 2+/), which has been shown to increase mmaTF-dependent procoagulant activity in whole blood, was used to stimulate similar pathways in our experiment. We spiked human citrated whole blood with HgCl/sub 2/ and Humulin-N/sup /spl reg// (HN) at final concentrations of control, 0.02 U/mL HN, 0.004 U/mL HN, 0.002 U/mL HN, 0.005% HgCl/sub 2/, 0.02 U/mL HN:0.005% HgCl/sub 2/, 0.004 U/mL HN:0.005% HgCl/sub 2/, and 0.002 U/mL HN:0.005% HgCl/sub 2/. The samples (n=12) were incubated at 37/spl deg/C for 10 minutes and recalcified with CaCl/sub 2/ to initiate clotting. The clotting time (CT) was measured with an Amelung KC4A Micro. The results indicate that HN by itself has no effect on CT. However, each concentration of HN:HgCl/sub 2/ exhibited a shorter CT than either HN or HgCl/sub 2/ alone (p<0.02 in each case). Studies with bovine pancreas insulin yielded similar results. This data indicates that insulin is stimulating mmaTF activity, and warrants the implementation of coagulation monitoring protocols for intensive insulin therapy.
Sponsorship	Schering Plough Becton Dickinson Boston Scientific Reva
Starting Page	251
Ending Page	252
File Size	184893
Page Count	2
File Format	PDF
ISBN	0780377672
DOI	10.1109/NEBC.2003.1216089
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2003-03-22
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Insulin Biomembranes Coagulation Blood Medical treatment Diabetes Cardiovascular diseases In vitro Mercury (metals) Humans
Content Type	Text
Resource Type	Article

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