NDLI: Effects of Al(III) and Nano-<formula formulatype=inline><tex Notation=TeX>${rm Al}_{13}$</tex></formula> on Aldehyde Dehydrogenase Activity on Reduced Graphene Oxide Modified Electrode

Content Provider	IEEE Xplore Digital Library
Author	Li Li Nan-Nan Zhang Xiao-Ling Ma Chong-Zheng Xu Hai-Yan Wei Xu-Jie Yang Xiao-Di Yang
Copyright Year	2001
Abstract	In this paper, electrochemical tests are applied to investigate the effects of Al(III) and nano-sized tridecameric aluminum polycation (nano- Al₁₃) on aldehyde dehydrogenase (ALDH) activity, by using reduced graphene oxide chemically modified electrodes. The amperometric i-t curves for the oxidation of the enzymatically generated diphosphopyridine nucleotide-reduced disodium salt (NADH) are collected, and the ALDH activity is determined indirectly, depending on the changes of the oxidation current of NADH. The results show that Al(III) and nano-Al₁₃ exhibit effective inhibition on ALDH activity. The proposed mechanism of the effects is explored by means of various analytical techniques, such as fluorescence spectroscopy and circular dichroism. Based on the characterization methods mentioned above, the remarkable activity reduction of ALDH might be ascribed to the conformational changes of coenzyme nicotinamide adenine dinucleotide (NAD⁺) induced by different aluminum species.
Sponsorship	IEEE Sensors Council
Starting Page	314
Ending Page	320
Page Count	7
File Size	1770702
File Format	PDF
ISSN	1530437X
Volume Number	13
Issue Number	1
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrodes Aluminum Oxidation Biochemistry Fluorescence Nanobioscience spectroscopy ${\rm Al}_{13}$ Al(III) aldehyde dehydrogenase electrochemical method graphene
Content Type	Text
Resource Type	Article
Subject	Instrumentation Electrical and Electronic Engineering

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