NDLI: A Microfluidic Device for Caenorhabditis Elegans Based Chemical Testing

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Song, Pengfei Zhang, Weize Sobolevski, Alexandre Bernard, Kristine Hekimi, Siegfried Liu, Xinyu
Copyright Year	2014
Abstract	The nematode worm Caenorhabditis elegans has been employed as a popular model organism in many fields of biological research. In this paper, we present an easy-to-use microfluidic device for facilitating C. elegans based chemical testing. The device is capable of housing single worms in microfluidic chambers and precisely adjusting the chamber’s chemical environment during experiments. Eight worms can be readily loaded into the chambers through separate loading channels in a quick and gentle manner. In addition, a custom-made software with a graphic user interface is also created for quantitative analysis of locomotion parameters (swimming frequency and bend amplitude) of the worms in response to chemical stimuli, thus greatly enhancing the efficiency of data collection. We perform proof-of-concept experiments using two chemicals, zinc ion (Zn2+) and glucose, which demonstrate the capability and effectiveness of the microfluidic device.
File Format	PDF
ISBN	9780791849590
DOI	10.1115/IMECE2014-39126
Volume Number	Volume 10: Micro- and Nano-Systems Engineering and Packaging
Conference Proceedings	ASME 2014 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2014-11-14
Publisher Place	Montreal, Quebec, Canada
Access Restriction	Subscribed
Subject Keyword	Data collection User interfaces Glucose Computer software Microfluidics Testing
Content Type	Text
Resource Type	Article

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