NDLI: Effect of Media Conductivity on High Frequency Dielectrophoretic Response

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Hadady, Hanieh Johnson, J. Wong Sage, R. Hiibel Emil, J. Geiger
Copyright Year	2014
Abstract	A method was developed for analyzing the high frequency dielectrophoresis (>20 MHz) response of microalgal cells with different lipid content on the basis of media conductivities. At these frequencies, the DEP response is expected to depend primarily on the dielectric properties of the cytoplasm. The medium used to perform DEP experiments has an important influence on their outcome since all properties are measured in relation to the medium, therefore its properties must be tightly controlled. This method is label free and only uses electric fields, and thus it is suitable for many other applications in the medical field. Chlamydomonas reinhardtii cells were cultured in regular medium and then the same cells were cultured under nitrogen-free conditions to accumulate neutral (non-polar) lipids. To measure the upper crossover frequency, two thin, needle shaped electrodes (100 nm thick) were patterned onto a glass slide using standard micro-technology processes and the motion of the cells was observed as an AC signal was swept from 20–80 MHz at ∼30Vpp (Volt peak to peak). Cells were injected to the microelectrode array in different medium conductivities that were adjusted by sodium chloride. The upper crossover frequencies of N-free cells in different media conductivities were measured. It was found that when the solution conductivity was between 4 ± 2 μs/cm and 106 ± 1 μS/cm and the operating frequency was between 20 and 80 MHz, the upper crossover frequency of the microalgae increased as the medium conductivity increased. The lipid content was verified via fluorescence microscopy and MATLAB image processing.
File Format	PDF
ISBN	9780791849590
DOI	10.1115/IMECE2014-38423
Volume Number	Volume 10: Micro- and Nano-Systems Engineering and Packaging
Conference Proceedings	ASME 2014 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2014-11-14
Publisher Place	Montreal, Quebec, Canada
Access Restriction	Subscribed
Subject Keyword	Electrical conductivity Image processing Glass Fluorescence Nitrogen Electrodes Biomedicine Microscopy Sodium Thermal conductivity Needles Signals Dielectrophoresis Performance Electric fields Matlab
Content Type	Text
Resource Type	Article

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