NDLI: The herpes simplex virus-1 encoded glycoprotein B diverts HLA-DR into the exosome pathway.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Eis-Hübinger, Anna M. Koch, Norbert McLellan, Alexander D. Temme, Sebastian
Description	Country affiliation: Germany Author Affiliation: Temme S ( Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, Germany.)
Abstract	Neutralizing Abs play an important role for immunity against HSV-1 infection. This branch of the immune response is initiated by MHC class II Ag presentation and activation of T cell help. In this study, we show that the HSV-1 encoded glycoprotein B (gB) manipulates the class II processing pathway by perturbing endosomal sorting and trafficking of HLA-DR (DR) molecules. Expression of gB in the human melanoma cell line Mel JuSo results in formation of enlarged DR(+) intracellular vesicles. Costaining of the vesicles revealed the presence of DR, gB, and the late endosomal marker CD63. The lumen of these late endosomal membranes shows a variable content, containing either gB or CD63, or both CD63 and gB. gB targets DR molecules on their biosynthetic route, after the MHC class II invariant chain is released from the DR heterodimer. gB-DR complexes were detected in a post-Golgi compartment and in exosomes, but not on the cell surface. Interestingly, increasing expression of gB strongly elevated the amount of DR and CD63 released into the exosome pathway. In conclusion, this is a previously undescribed mode of viral immune evasion involving hijacking of DR from its normal transport route to the cell surface, followed by viral-mediated release of DR into the exosome pathway.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	1
Volume Number	184
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2010-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antigens, Cd Metabolism Exosomes Virology Hla-dr Antigens Herpes Simplex Immunology Immune Evasion Platelet Membrane Glycoproteins Viral Envelope Proteins Antigens, Cd63 Blotting, Western Cell Line, Tumor Flow Cytometry Herpesvirus 1, Human Immunoprecipitation Microscopy, Fluorescence Protein Transport Physiology Transfection Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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