NDLI: Biodegradable polyelectrolyte microcapsules: antigen delivery tools with Th17 skewing activity after pulmonary delivery.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	De Smedt, Stefaan Grooten, Johan De Koker, Stefaan Bogaert, Pieter Naessens, Thomas Demeester, Jo De Geest, Bruno G.
Description	Country affiliation: Belgium Author Affiliation: De Koker S ( Department of Biomedical Molecular Biology, Ghent University, Zwijnaarde, Belgium.)
Abstract	Because of their large surface area, the lungs appear an attractive route for noninvasive vaccine delivery, harboring the potential to induce local mucosal immune responses in addition to systemic immunity. To evoke adaptive immunity, Ags require the addition of adjuvants that not only enhance the strength of the immune response but also determine the type of response elicited. In this study, we evaluate the adjuvant characteristics of polyelectrolyte microcapsules (PEMs) consisting of the biopolymers dextran-sulfate and poly-L-arginine. PEMs form an entirely new class of microcapsules that are generated by the sequential adsorption of oppositely charged polymers (polyelectrolytes) onto a sacrificial colloidal template, which is subsequently dissolved leaving a hollow microcapsule surrounded by a thin shell. Following intratracheal instillation, PEMs were not only efficiently taken up by APCs but also enhanced their activation status. Pulmonary adaptive immune responses were characterized by the induction of a strongly Th17-polarized response. When compared with a mixture of soluble Ag with empty microcapsules, Ag encapsulation significantly enhanced the strength of this local mucosal response. Given their unique property to selectively generate Th17-polarized immune responses, PEMs may become of significant interest in the development of effective vaccines against fungal and bacterial species.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	1
Volume Number	184
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2010-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Absorbable Implants Adjuvants, Immunologic Administration & Dosage Capsules Interleukin-17 Immunology Lung Vaccination Chemical Synthesis Animals Antigen-presenting Cells Antigens Bronchoalveolar Lavage Dextran Sulfate Electrolytes Flow Cytometry Immunity, Mucosal Lymphocyte Activation Mice Mice, Inbred C57bl Peptides Reverse Transcriptase Polymerase Chain Reaction Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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