NDLI: Existence of CD8 -like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bonneau, Michel Urien, Céline Andrieu, Thibault Epardaud, Mathieu Crozat, Karine Contreras, Vanessa Alexandre, Yannick Schwartz-Cornil, Isabelle Savina, Ariel Hope, Jayne Guiton, Rachel Vu Manh, Thien-Phong Jouneau, Luc Bertho, Nicolas Amigorena, Sebastian Dalod, Marc
Description	Country affiliation: France Author Affiliation: Contreras V ( Virologie et Immunologie Moléculaires UR892, Institut National de Recherche Agronomique, Domaine de Vilvert, Jouy-en-Josas, France.)
Abstract	The mouse lymphoid organ-resident CD8alpha(+) dendritic cell (DC) subset is specialized in Ag presentation to CD8(+) T cells. Recent evidence shows that mouse nonlymphoid tissue CD103(+) DCs and human blood DC Ag 3(+) DCs share similarities with CD8alpha(+) DCs. We address here whether the organization of DC subsets is conserved across mammals in terms of gene expression signatures, phenotypic characteristics, and functional specialization, independently of the tissue of origin. We study the DC subsets that migrate from the skin in the ovine species that, like all domestic animals, belongs to the Laurasiatheria, a distinct phylogenetic clade from the supraprimates (human/mouse). We demonstrate that the minor sheep CD26(+) skin lymph DC subset shares significant transcriptomic similarities with mouse CD8alpha(+) and human blood DC Ag 3(+) DCs. This allowed the identification of a common set of phenotypic characteristics for CD8alpha-like DCs in the three mammalian species (i.e., SIRP(lo), CADM1(hi), CLEC9A(hi), CD205(hi), XCR1(hi)). Compared to CD26(-) DCs, the sheep CD26(+) DCs show 1) potent stimulation of allogeneic naive CD8(+) T cells with high selective induction of the Ifngamma and Il22 genes; 2) dominant efficacy in activating specific CD8(+) T cells against exogenous soluble Ag; and 3) selective expression of functional pathways associated with high capacity for Ag cross-presentation. Our results unravel a unifying definition of the CD8alpha(+)-like DCs across mammalian species and identify molecular candidates that could be used for the design of vaccines applying to mammals in general.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	6
Volume Number	185
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2010-09-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antigens, Cd8 Biosynthesis Conserved Sequence Dendritic Cells Immunology Gene Expression Profiling Lymph Cytology Animals Physiology Cells, Cultured Metabolism Dipeptidyl Peptidase 4 Immune Tolerance Mice Sheep, Domestic Skin Species Specificity Comparative Study Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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