NDLI: Systemic circulation and bone recruitment of osteoclast precursors tracked by using fluorescent imaging techniques.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kikuta, Junichi Chino, Takenao Klauschen, Frederick Tomura, Michio Ishii, Masaru Kotani, Manato Kobayashi, Yasuhiro Kanagawa, Osami Miyawaki, Atsushi Yasuda, Hisataka Tamai, Katsuto
Description	Country affiliation: Japan Author Affiliation: Kotani M ( Laboratory of Cellular Dynamics, World Premier International Research Center Initiative-Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.)
Abstract	Osteoclasts are bone-resorbing polykaryons differentiated from monocyte/macrophage-lineage hematopoietic precursors. It remains unclear whether osteoclasts originate from circulating blood monocytes or from bone tissue-resident precursors. To address this question, we combined two different experimental procedures: 1) shared blood circulation 'parabiosis' with fluorescently labeled osteoclast precursors, and 2) photoconversion-based cell tracking with a Kikume Green-Red protein (KikGR). In parabiosis, CX(3)CR1-EGFP knock-in mice in which osteoclast precursors were labeled with EGFP were surgically connected with wild-type mice to establish a shared circulation. Mature EGFP(+) osteoclasts were found in the bones of the wild-type mice, indicating the mobilization of EGFP(+) osteoclast precursors into bones from systemic circulation. Receptor activator for NF-κB ligand stimulation increased the number of EGFP(+) osteoclasts in wild-type mice, suggesting that this mobilization depends on the bone resorption state. Additionally, KikGR(+) monocytes (including osteoclast precursors) in the spleen were exposed to violet light, and 2 d later we detected photoconverted 'red' KikGR(+) osteoclasts along the bone surfaces. These results indicate that circulating monocytes from the spleen entered the bone spaces and differentiated into mature osteoclasts during a certain period. The current study used fluorescence-based methods clearly to demonstrate that osteoclasts can be generated from circulating monocytes once they home to bone tissues.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	2
Volume Number	190
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2013-01-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bone And Bones Metabolism Cell Tracking Microscopy, Fluorescence, Multiphoton Osteoclasts Stem Cells Animals Cell Movement Cross Circulation Mice Mice, Transgenic Cytology Parabiosis Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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