NDLI: IL-21 enhances the activity of the TLR-MyD88-STAT3 pathway but not the classical TLR-MyD88-NF-κB pathway in human B cells to boost antibody production.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liu, Bi-Sheng Stoop, Jeroen N. Toes, Rene E. M. Huizinga, Tom W.
Description	Country affiliation: Netherlands Author Affiliation: Liu BS ( Department of Rheumatology, Leiden University Medical Center, 2300RC Leiden, The Netherlands.)
Abstract	Both IL-21 and TLR agonists are important regulators of B cell responses, and the combination of IL-21 and TLR stimulation results in increased Ab production. However, it is not clear yet how IL-21 interacts with TLR signaling in B cells. In this study, we show that IL-21 enhances TLR-induced IgG production, whereas it has no effect on TLR-induced IL-6 production by human B cell cultures. These observations are explained by the finding that IL-21 augments TLR-induced IgG production via the TLR-MyD88-STAT3 pathway but not the classical TLR-MyD88-NF-κB pathway. We further demonstrate that stimulation of human B cells with IL-21 and TLR7/8 or TLR9 agonists increases the phosphorylation of STAT3, whereas the activation of NF-κB is not affected. Interestingly, like IL-21, IL-10 in combination with TLR signaling also enhances phosphorylation of STAT3, resulting in an increase of IgG production. Hence, IL-21 and IL-10 increase the activity of the TLR-MyD88-STAT3 pathway in human B cells via enhancing the phosphorylation of STAT3 for Ab production.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	8
Volume Number	191
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2013-10-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	B-lymphocytes Metabolism Interleukin-10 Interleukins Myeloid Differentiation Factor 88 Stat3 Transcription Factor Antibody Formation Immunology Cells, Cultured Immunoglobulin G Biosynthesis Immunoglobulin M Interleukin-6 Agonists Lymphocyte Activation Nf-kappa B Phosphorylation Repressor Proteins Signal Transduction Toll-like Receptor 7 Toll-like Receptor 8 Toll-like Receptor 9 Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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